Antimicrobial Resistance and Genomic Characterization of Six New Sequence Types in Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates from Pakistan.
Antimicrobial Resistance and Genomic Characterization of Six New Sequence Types in Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates from Pakistan.
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DOI：
10.3390/antibiotics10111386
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发表时间：
2021-11-12
期刊：
影响因子：
--
通讯作者：
Andleeb S
中科院分区：
文献类型：
--
作者：
Irum S;Naz K;Ullah N;Mustafa Z;Ali A;Arslan M;Khalid K;Andleeb S
铜绿假单胞菌（P. aeruginosa）是一种与多种感染相关的主要细菌病原体，具有高死亡率。大多数临床铜绿假单胞菌分离株属于有限数量的遗传亚群，其特征在于通过多位点序列分型（MLST）方案的多个管家基因序列（通常5-7个）。铜绿假单胞菌新的多药耐药（MDR）序列类型（ST）的出现和传播引起了严重的临床问题。我们对从巴基斯坦一家三级医院实验室收集的MDR铜绿假单胞菌分离株进行了全基因组测序，发现6株分离株属于6个独特的MLST等位基因谱。将基因组提交至PubMLST数据库，并将新的ST编号（ST 3493、ST 3494、ST 3472、ST 3489、ST 3491和ST 3492）分配给相应的等位基因组合。MLST和基于核心基因组的系统发育分析证实了这些分离株的分歧，并将它们定位在不同的分支。新ST分离株的耐药基因组分析显示存在基因blaOXA-50、blaPAO、blaPDC、blaVIM-2、aph（3′）-IIb、aac（6′）-II、aac（3）-Id、fosA、catB 7、dfrB 2、crpP、merP以及染色体基因中的许多错义和移码突变，这些基因赋予对各种抗假单胞菌抗生素的耐药性。exoS、exoT、pvdE、rhlI、rhlR、lasA、lasB、lasI和lasR基因是ST新分离株中最常见的毒力相关基因。不同的基因型特征揭示了这些新克隆通过影响抗生素耐药性、群体感应和生物膜形成的基因中的各种突变来适应各种感染。需要密切监测这些耐药病原体和监测机制，以减少它们向巴基斯坦医疗设施的传播。我们相信，这些菌株可以作为参考菌株，为未来的比较分析属于相同的ST的菌株。
Pseudomonas aeruginosa (P. aeruginosa) is a major bacterial pathogen associated with a variety of infections with high mortality rates. Most of the clinical P. aeruginosa isolates belong to a limited number of genetic subgroups characterized by multiple housekeeping genes’ sequences (usually 5–7) through the Multi-Locus Sequence Typing (MLST) scheme. The emergence and dissemination of novel multidrug-resistant (MDR) sequence types (ST) in P. aeruginosa pose serious clinical concerns. We performed whole-genome sequencing on a cohort (n = 160) of MDR P. aeruginosa isolates collected from a tertiary care hospital lab in Pakistan and found six isolates belonging to six unique MLST allelic profiles. The genomes were submitted to the PubMLST database and new ST numbers (ST3493, ST3494, ST3472, ST3489, ST3491, and ST3492) were assigned to the respective allele combinations. MLST and core-genome-based phylogenetic analysis confirmed the divergence of these isolates and positioned them in separate branches. Analysis of the resistome of the new STs isolates revealed the presence of genes blaOXA-50, blaPAO, blaPDC, blaVIM-2, aph(3′)-IIb, aac(6′)-II, aac(3)-Id, fosA, catB7, dfrB2, crpP, merP and a number of missense and frame-shift mutations in chromosomal genes conferring resistance to various antipseudomonal antibiotics. The exoS, exoT, pvdE, rhlI, rhlR, lasA, lasB, lasI, and lasR genes were the most prevalent virulence-related genes among the new ST isolates. The different genotypic features revealed the adaptation of these new clones to a variety of infections by various mutations in genes affecting antimicrobial resistance, quorum sensing and biofilm formation. Close monitoring of these antibiotic-resistant pathogens and surveillance mechanisms needs to be adopted to reduce their spread to the healthcare facilities of Pakistan. We believe that these strains can be used as reference strains for future comparative analysis of isolates belonging to the same STs.
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影响因子：
4.9
作者：
de la Rosa, Jose Manuel Ortiz;Nordmann, Patrice;Poirel, Laurent
通讯作者：
Poirel, Laurent
影响因子：
5.2
作者：
Bianconi, Irene;D'Arcangelo, Silvia;Jousson, Olivier
通讯作者：
Jousson, Olivier
影响因子：
14.2
作者：
Magiorakos, A. -P.;Srinivasan, A.;Monnet, D. L.
通讯作者：
Monnet, D. L.
基因组分析表明，铜绿假单胞菌的毒力是组合。
影响因子：
12.3
作者：
Lee DG;Urbach JM;Wu G;Liberati NT;Feinbaum RL;Miyata S;Diggins LT;He J;Saucier M;Déziel E;Friedman L;Li L;Grills G;Montgomery K;Kucherlapati R;Rahme LG;Ausubel FM
通讯作者：
Ausubel FM
影响因子：
5.2
作者：
Freschi L;Jeukens J;Kukavica-Ibrulj I;Boyle B;Dupont MJ;Laroche J;Larose S;Maaroufi H;Fothergill JL;Moore M;Winsor GL;Aaron SD;Barbeau J;Bell SC;Burns JL;Camara M;Cantin A;Charette SJ;Dewar K;Déziel É;Grimwood K;Hancock RE;Harrison JJ;Heeb S;Jelsbak L;Jia B;Kenna DT;Kidd TJ;Klockgether J;Lam JS;Lamont IL;Lewenza S;Loman N;Malouin F;Manos J;McArthur AG;McKeown J;Milot J;Naghra H;Nguyen D;Pereira SK;Perron GG;Pirnay JP;Rainey PB;Rousseau S;Santos PM;Stephenson A;Taylor V;Turton JF;Waglechner N;Williams P;Thrane SW;Wright GD;Brinkman FS;Tucker NP;Tümmler B;Winstanley C;Levesque RC
通讯作者：
Levesque RC