Effect of plagiochin E, an antifungal macrocyclic bis(bibenzyl), on cell wall chitin synthesis in Candida albicans
Effect of plagiochin E, an antifungal macrocyclic bis(bibenzyl), on cell wall chitin synthesis in Candida albicans
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plagiochin E（一种抗真菌大环双（联苄））对白色念珠菌细胞壁几丁质合成的影响
DOI：
10.1111/j.1745-7254.2008.00900.x
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发表时间：
2008-12
期刊：
影响因子：
--
通讯作者：
Lou, Hong-xiang
中科院分区：
文献类型：
--
作者：
Cheng, Ai-xia;Sun, Ling-mei;Wu, Xiu-zhen;Lou, Hong-xiang
摘要：研究从地茅中分离的抗真菌大环双（联苯）物plagiochin E （PLE）对白色念珠菌细胞壁几丁质合成的影响。方法：从细胞水平和分子水平研究PLE对白色念珠菌几丁质合成的影响。首先在透射电镜下观察其超微结构变化。其次，以6- o -丹酰- n -乙酰氨基葡萄糖为荧光底物，检测PLE对体外几丁质合成酶（Chs）活性的影响，并通过球质体再生法检测其对几丁质原位合成的影响。最后采用实时RT-PCR检测其对Chs基因（Chs）表达的影响。结果：透射电镜观察显示，白色念珠菌细胞壁结构被严重破坏，提示PLE的抗真菌活性与其对细胞壁的作用有关。酶促实验和球质体再生表明，PLE在体外和原位均能抑制几丁质合成。PCR结果显示，PLE显著下调CHS1的表达，上调CHS2和CHS3的表达。由于不同的Chs在转录和翻译后的不同阶段受到调控，下调CHS1会降低CHS1的水平并抑制其活性，而PLE对Chs2和Chs3的抑制作用可能在翻译后水平或通过抑制酶活性中心来实现。结论：PLE的抗真菌活性可能与其抑制白色念珠菌细胞壁几丁质合成有关。
AbstractAim:To investigate the effect of plagiochin E (PLE), an antifungal macrocyclic bis(bibenzyl) isolated from liverwort Marchantia polymorpha L, on cell wall chitin synthesis in Candida albicans.Methods:The effect of PLE on chitin synthesis in Candida albicans was investigated at the cellular and molecular levels. First, the ultrastructural changes were observed under transmission electron microscopy (TEM). Second, the effects of PLE on chitin synthetase (Chs) activities in vitro were assayed using 6-O-dansyl-N-acetylglucosamine as a fluorescent substrate, and its effect on chitin synthesis in situ was assayed by spheroplast regeneration. Finally, real-time RT–PCR was performed to assay its effect on the expression of Chs genes (CHS).Results:Observation under TEM showed that the structure of the cell wall in Candida albicans was seriously damaged, which suggested that the antifungal activity of PLE was associated with its effect on the cell wall. Enzymatic assays and spheroplast regeneration showed that PLE inhibited chitin synthesis in vitro and in situ. The results of the PCR showed that PLE significantly downregulated the expression of CHS1, and upregulated the expression of CHS2 and CHS3. Because different Chs is regulated at different stages of transcription and post-translation, the downregulation of CHS1 would decrease the level of Chs1 and inhibit its activity, and the inhibitory effects of PLE on Chs2 and Chs3 would be at the post-translational level or by the inhibition on the enzyme-active center.Conclusion:These results indicate that the antifungal activity of PLE would be attributed to its inhibitory effect on cell wall chitin synthesis in Candida albicans.
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影响因子：
4.4
作者：
K. Bahmed;F. Quilès;M. Wathier;R. Bonaly;S. Benallaoua;B. Pucci;J. Coulon
通讯作者：
K. Bahmed;F. Quilès;M. Wathier;R. Bonaly;S. Benallaoua;B. Pucci;J. Coulon
DOI：
10.7164/antibiotics.50.177
发表时间：
1997-02
期刊：
The Journal of antibiotics
影响因子：
--
作者：
L. Carrano;P. Tavecchia;F. Sponga;F. Spreafico
通讯作者：
L. Carrano;P. Tavecchia;F. Sponga;F. Spreafico
影响因子：
4.9
作者：
T. Parkinson;D. Falconer;C. Hitchcock
通讯作者：
T. Parkinson;D. Falconer;C. Hitchcock
影响因子：
2.1
作者：
T. Hashimoto;H. Irita;S. Takaoka;Masami Tanaka;Y. Asakawa
通讯作者：
T. Hashimoto;H. Irita;S. Takaoka;Masami Tanaka;Y. Asakawa
DOI：
10.1046/j.1432-1327.1998.2560419.x
发表时间：
1998-09-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Cos, T;Ford, RA;Roncero, C
通讯作者：
Roncero, C