Thaxtomin A affects CESA-complex density, expression of cell wall genes, cell wall composition, and causes ectopic lignification in Arabidopsis thaliana seedlings.
Thaxtomin A affects CESA-complex density, expression of cell wall genes, cell wall composition, and causes ectopic lignification in Arabidopsis thaliana seedlings.
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DOI：
10.1093/jxb/ern344
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发表时间：
2009
影响因子：
6.9
通讯作者：
Scheible WR
中科院分区：
文献类型：
--
作者：
Bischoff V;Cookson SJ;Wu S;Scheible WR
Thaxtomin A 是一种由真细菌链霉菌产生的植物毒素，被怀疑充当天然纤维素合成抑制剂。对用 thaxtomin A 处理的拟南芥幼苗进行的新的化学、分子和显微分析结果证实了这一观点。细胞壁分析表明，thaxtomin A 减少了结晶纤维素，并增加了细胞壁中的果胶和半纤维素。 thaxtomin A 处理还改变了参与初级和次级纤维素合成的基因以及与果胶代谢和细胞壁重塑相关的基因的表达，其方式与异恶苯几乎相同。此外，它还诱导几种防御相关基因的表达并导致胼胝质沉积。纤维素合成的缺陷导致拟南芥中异位木质化表型，并且表明木质化也由thaxtomin A引发，尽管其模式与异恶苯不同。转盘共聚焦显微镜进一步揭示，thaxtomin A 会消耗质膜上的纤维素合酶复合物，并导致这些颗粒在与微管相关的小室中积累。这些结果为 thaxtomin A 对纤维素合成具有强烈影响提供了新的明确证据，从而表明这是其主要作用方式。
Thaxtomin A, a phytotoxin produced by Streptomyces eubacteria, is suspected to act as a natural cellulose synthesis inhibitor. This view is confirmed by the results obtained from new chemical, molecular, and microscopic analyses of Arabidopsis thaliana seedlings treated with thaxtomin A. Cell wall analysis shows that thaxtomin A reduces crystalline cellulose, and increases pectins and hemicellulose in the cell wall. Treatment with thaxtomin A also changes the expression of genes involved in primary and secondary cellulose synthesis as well as genes associated with pectin metabolism and cell wall remodelling, in a manner nearly identical to isoxaben. In addition, it induces the expression of several defence-related genes and leads to callose deposition. Defects in cellulose synthesis cause ectopic lignification phenotypes in A. thaliana, and it is shown that lignification is also triggered by thaxtomin A, although in a pattern different from isoxaben. Spinning disc confocal microscopy further reveals that thaxtomin A depletes cellulose synthase complexes from the plasma membrane and results in the accumulation of these particles in a small microtubule-associated compartment. The results provide new and clear evidence for thaxtomin A having a strong impact on cellulose synthesis, thus suggesting that this is its primary mode of action.
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影响因子：
7.2
作者：
Caño-Delgado, A;Penfield, S;Bevan, M
通讯作者：
Bevan, M
DOI：
10.1073/pnas.0706569104
发表时间：
2007-09-25
影响因子：
11.1
作者：
Desprez, Thierry;Juraniec, Michal;Vernhettes, Samantha
通讯作者：
Vernhettes, Samantha
影响因子：
7.2
作者：
Bernal, Adriana Jimena;Jensen, Jakob Kruger;Willats, William G. T.
通讯作者：
Willats, William G. T.
影响因子：
11.6
作者：
Brown, DM;Zeef, LAH;Turner, SR
通讯作者：
Turner, SR
影响因子：
7.4
作者：
Desprez, T;Vernhettes, S;Höfte, H
通讯作者：
Höfte, H