The Evolution of Widespread Recombination Suppression on the Dwarf Hamster (Phodopus) X Chromosome.
The Evolution of Widespread Recombination Suppression on the Dwarf Hamster (Phodopus) X Chromosome.
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DOI：
10.1093/gbe/evac080
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发表时间：
2022-05-31
影响因子：
3.3
通讯作者：
Good, Jeffrey M.
中科院分区：
文献类型：
--
作者：
Moore, Emily C.;Thomas, Gregg W. C.;Mortimer, Sebastian;Kopania, Emily E. K.;Hunnicutt, Kelsie E.;Clare-Salzler, Zachary J.;Larson, Erica L.;Good, Jeffrey M.
兽目哺乳动物的X染色体在远亲物种中表现出很强的保守性，限制了对塑造性染色体进化的独特选择过程的深入了解。我们构建了一个染色体规模的从头基因组组装的西伯利亚矮仓鼠（Phodopus sungorus），一个物种的报告显示广泛的重组抑制整个手臂的X染色体。结合物理基因组组装基于鸟枪和长距离邻近连接测序与密集的遗传图谱，我们检测到广泛的抑制女性重组跨越65%的Photopus X染色体。这个抑制重组的区域可能对应于Xp臂，其先前已被证明是高度异染色质的。使用额外的测序数据，从两个密切相关的物种（P. campbelli和P. roborovskii），我们表明，重组抑制Xp似乎是独立的主要结构重排。被抑制的Xp臂富集了几个转座因子家族和去富集主要在胎盘中表达的基因，但在其他方面表现出类似的基因密度，表达模式，和分子进化率相比，重组Xq臂。Photopus Xp基因的内容和顺序也广泛保守相对于更远的相关大鼠X染色体。这些数据表明，广泛的抑制重组可能已经演变通过瞬时诱导的兼性异染色质的Phodopus Xp臂没有重大变化的染色体结构或遗传内容。因此，到目前为止，重组景观的实质性变化对这些物种的X连锁分子进化模式产生了相对微妙的影响。
The X chromosome of therian mammals shows strong conservation among distantly related species, limiting insights into the distinct selective processes that have shaped sex chromosome evolution. We constructed a chromosome-scale de novo genome assembly for the Siberian dwarf hamster (Phodopus sungorus), a species reported to show extensive recombination suppression across an entire arm of the X chromosome. Combining a physical genome assembly based on shotgun and long-range proximity ligation sequencing with a dense genetic map, we detected widespread suppression of female recombination across ∼65% of the Phodopus X chromosome. This region of suppressed recombination likely corresponds to the Xp arm, which has previously been shown to be highly heterochromatic. Using additional sequencing data from two closely related species (P. campbelli and P. roborovskii), we show that recombination suppression on Xp appears to be independent of major structural rearrangements. The suppressed Xp arm was enriched for several transposable element families and de-enriched for genes primarily expressed in placenta, but otherwise showed similar gene densities, expression patterns, and rates of molecular evolution when compared to the recombinant Xq arm. Phodopus Xp gene content and order was also broadly conserved relative to the more distantly related rat X chromosome. These data suggest that widespread suppression of recombination has likely evolved through the transient induction of facultative heterochromatin on the Phodopus Xp arm without major changes in chromosome structure or genetic content. Thus, substantial changes in the recombination landscape have so far had relatively subtle influences on patterns of X-linked molecular evolution in these species.
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影响因子：
3.7
作者：
Chapman JA;Ho I;Sunkara S;Luo S;Schroth GP;Rokhsar DS
通讯作者：
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DOI：
10.1073/pnas.1416622112
发表时间：
2015-02-17
影响因子：
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作者：
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通讯作者：
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