Comparative analysis of piRNA sequences, targets and functions in nematodes.
Comparative analysis of piRNA sequences, targets and functions in nematodes.
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DOI：
10.1080/15476286.2022.2149170
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发表时间：
2022-01
期刊：
影响因子：
4.1
通讯作者：
Tang, Wen
中科院分区：
文献类型：
--
作者：
Pastore, Benjamin;Hertz, Hannah L.;Tang, Wen
Piwi蛋白和Piwi相互作用RNA（piRNA）最为人所知的是它们在抑制转座子和促进生育方面的作用。然而，皮尔纳的生物发生及其作用机制在远亲物种之间存在很大差异。为了更好地理解piRNA的进化，我们表征了C. briggsae是模式生物C.优雅我们的分析在C.布里格塞C. briggsae与C.虽然这两个物种的基因组结构相似，但它们都能驱动皮尔纳的表达。通过研究Piwi介导的二级小RNA的产生，我们鉴定了一组蛋白质编码基因，它们是进化上保守的皮尔纳靶标。与C.在线虫中，靶向核糖体RNA或组蛋白转录物的小RNA在C.布里格萨·皮维变种人相反，我们发现具有很少内含子的转录物倾向于小RNA过度扩增。我们的工作突出了线虫皮尔纳途径的进化保守性和差异性，并提供了对其在内源基因调控中作用的见解。
Piwi proteins and Piwi-interacting RNAs (piRNAs) are best known for their roles in suppressing transposons and promoting fertility. Yet piRNA biogenesis and its mechanisms of action differ widely between distantly related species. To better understand the evolution of piRNAs, we characterized the piRNA pathway in C. briggsae, a sibling species of the model organism C. elegans. Our analyses define 25,883 piRNA producing-loci in C. briggsae. piRNA sequences in C. briggsae are extremely divergent from their counterparts in C. elegans, yet both species adopt similar genomic organization that drive piRNA expression. By examining production of Piwi-mediated secondary small RNAs, we identified a set of protein-coding genes that are evolutionarily conserved piRNA targets. In contrast to C. elegans, small RNAs targeting ribosomal RNAs or histone transcripts are not hyper-accumulated in C. briggsae Piwi mutants. Instead, we found that transcripts with few introns are prone to small RNA overamplification. Together our work highlights evolutionary conservation and divergence of the nematode piRNA pathway and provides insights into its role in endogenous gene regulation.
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模因套件：用于发现和搜索的工具。
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