Transcriptome and volatile compounds profiling analyses provide insights into the molecular mechanism underlying the floral fragrance of tree peony
Transcriptome and volatile compounds profiling analyses provide insights into the molecular mechanism underlying the floral fragrance of tree peony
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转录组和挥发性化合物分析可深入了解牡丹花香的分子机制
DOI：
10.1016/j.indcrop.2021.113286
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发表时间：
2021-04
影响因子：
5.9
通讯作者：
Shi Qianqian
中科院分区：
文献类型：
--
作者：
Zhang Yue;Li Chenyao;Wang Shu;Yuan Meng;Li Bingjie;Niu Lixin;Shi Qianqian
牡丹（Paeonia suffruticosaAndr.）是世界各地流行的观赏植物，以其独特的大而芬芳的花朵而闻名。然而，牡丹花香合成的潜在分子机制仍知之甚少。本研究对四种野生牡丹：奥氏芍药、洛氏芍药、德拉瓦氏芍药和黄芍药材进行了综合挥发性分析和RNA测序。通过气相色谱-质谱 (GC-MS) 分析共鉴定出 67 种花香挥发物。萜烯是P中最丰富的挥发物。奥斯蒂和P。 rockii中苯类化合物含量相对较高。德拉瓦伊和P.黄体。转录组数据揭示了 17,967 个差异表达基因 (DEG)，其中 116 个和 147 个分别与萜烯和苯类化合物的积累有关。具体而言，1-脱氧-d-木酮糖5-磷酸合酶、香叶基焦磷酸合酶、法呢基焦磷酸合酶和萜烯合酶可能是P中花萜生物合成的主要调节因子。奥斯蒂和P。 rockii，而 3-脱氧-7-磷酸庚酮酸合酶、苯丙氨酸解氨酶、肉桂酸：CoA 连接酶和短链脱氢酶/还原酶可能控制 P 中苯类化合物的产生。德拉瓦伊和P.黄体。综上所述，本研究结果揭示了不同牡丹品种香气变异的分子机制，也为研究牡丹花香形成提供了宝贵的资源。
Tree peony (Paeonia suffruticosaAndr.) is a popular ornamental plant around the world, which is known for its distinctively large and fragrant flowers. However, the underlying molecular mechanism of floral fragrance synthesis in tree peony remains poorly understood. In this study, integrative volatile profiling and RNA sequencing were conducted in four wild tree peony species:Paeonia ostii,Paeonia rockii,Paeonia delavayiandPaeonia lutea. A total of 67 floral volatiles were identified through gas chromatography-mass spectrometry (GC–MS) analysis. Terpenes were most abundant volatiles inP. ostiiandP. rockii, while benzenoids content were relatively higher inP. delavayiandP. lutea. Transcriptome data revealed 17,967 differential expression genes (DEGs), 116 and 147 of which were found be related to the accumulation of terpenes and benzenoids, respectively. Specifically, 1-deoxy-d-xylulose 5-phosphate synthase, geranyl pyrophosphate synthase, farnesyl pyrophosphate synthase and terpene synthase may be the major regulators of floral terpenes biosynthesis inP. ostiiandP. rockii, whilst 3-deoxy-7-phosphoheptulonate synthase, phenylalanine ammonialyase, cinnamate: CoA ligase and short-chain dehydrogenases/reductases may control the production of benzenoids production inP. delavayiandP. lutea. Taken together, results from this study sheds light on the molecular mechanism of fragrance variation among different tree peony species and also provides a valuable resource to investigate floral fragrance formation in tree peony.
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影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
DOI：
10.3390/molecules22081364
发表时间：
2017-08-17
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Shi Q;Li L;Zhang X;Luo J;Li X;Zhai L;He L;Zhang Y
通讯作者：
Zhang Y
DOI：
10.1073/pnas.1211001109
发表时间：
2012-10-02
影响因子：
11.1
作者：
Qualley, Anthony V.;Widhalm, Joshua R.;Dudareva, Natalia
通讯作者：
Dudareva, Natalia
影响因子：
3.7
作者：
Shi Q;Zhou L;Wang Y;Li K;Zheng B;Miao K
通讯作者：
Miao K
影响因子：
7.2
作者：
Long, Michael C.;Nagegowda, Dinesh A.;Dudareva, Natalia
通讯作者：
Dudareva, Natalia