Pluripotency-associated genes in human nasopharyngeal carcinoma CNE-2 cells are reactivated by a unique epigenetic sub-microenvironment.
Pluripotency-associated genes in human nasopharyngeal carcinoma CNE-2 cells are reactivated by a unique epigenetic sub-microenvironment.
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人鼻咽癌 CNE-2 细胞中的多能相关基因被独特的表观遗传亚微环境重新激活。
DOI：
10.1186/1471-2407-10-68
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发表时间：
2010-02-25
期刊：
影响因子：
3.8
通讯作者：
Liu Q
中科院分区：
文献类型：
--
作者：
Cao JX;Cui YX;Long ZJ;Dai ZM;Lin JY;Liang Y;Zheng FM;Zeng YX;Liu Q
There is increasing evidence that cancers contain their own stem-like cells, and particular attention has been paid to one subset of cancer-stem cells termed side population (SP). Stem cells under normal physical conditions are tightly controlled by their microenvironment, however, the regulatory role of the microenvironment surrounding cancer stem cells is not well characterized yet. In this study we found that the phenotype of SP can be "generated" by macrophage-like cells under conditioned culture. Furthermore the gene regulation pathway involved in cellular reprogramming process was investigated. The selection and identification of SP in 50 CNE-2 single cell clones were performed by flow cytometry. The transwell assay and immunofluorescence staining were used to measure migration and cancer stem cell characters of non-SP single clone cells cultured with conditioned medium respectively. The subtraction suppression hybridization (SSH) technique and northern blotting analysis was applied to explore the pluripotency-associated genes under a unique epigenetic sub-microenvironment. Among 50 clones, only one did not possess SP subpopulation while others did. The non-SP cells induced by macrophage-like cells showed more aggressive characters, which increased cell migration compared with the control cells and showed some fraction of SP phenotype. These cells expressed distinguished level of pluripotency-associated genes such as ADP-ribosylation factor-like 6 interacting protein (ARMER), poly (rC) binding protein 1 (PCBP1) and pyruvate dehydrogenase E1-β subunit (PDHB) when subjected to the environment. To our knowledge, this is the first study to demonstrate that non-SP single-clone cells can be induced to generate a SP phenotype when they are cultured with conditioned medium of macrophage-like cells, which is associated with the reactivation of pluripotency-associated genes.
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人肝癌细胞系中具有逐步转移潜力的侧群细胞的鉴定
DOI：
10.1007/s00432-008-0407-1
发表时间：
2008-11-01
影响因子：
3.6
作者：
Shi, Guo-Ming;Xu, Yang;Wu, Zhi-Quan
通讯作者：
Wu, Zhi-Quan
影响因子：
11.2
作者：
Chiba, Tetsuhiro;Miyagi, Satoru;Iwama, Atsushi
通讯作者：
Iwama, Atsushi
影响因子：
4.8
作者：
Mitsutake, Norisato;Iwao, Atsuhiko;Yamashita, Shunichi
通讯作者：
Yamashita, Shunichi
影响因子：
64.5
作者：
Kim, CFB;Jackson, EL;Jacks, T
通讯作者：
Jacks, T
影响因子：
64.8
作者：
Singh, SK;Hawkins, C;Dirks, PB
通讯作者：
Dirks, PB