Genome-wide analysis of MYB transcription factors of Vaccinium corymbosum and their positive responses to drought stress.
Genome-wide analysis of MYB transcription factors of Vaccinium corymbosum and their positive responses to drought stress.
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伞房越桔MYB转录因子的全基因组分析及其对干旱胁迫的响应。
DOI：
10.1186/s12864-021-07850-5
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发表时间：
2021-07-22
期刊：
影响因子：
4.4
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Wang A;Liang K;Yang S;Cao Y;Wang L;Zhang M;Zhou J;Zhang L
蓝莓（Vaccinium corymbosum L.）是果实中黄酮类化合物含量较高的重要品种。蓝莓是一种多年生灌木，具有根系浅、易受干旱胁迫的特点。MYB转录因子广泛参与了植物对非生物胁迫的响应，但MYB家族在蓝莓干旱胁迫响应中的作用尚不清楚。本研究基于干旱胁迫下蓝莓的基因组数据，对蓝莓中VcMYBs进行了系统分析，包括系统发育关系、差异表达基因（DEG）鉴定、表达谱分析、保守基序、表达相关性和蛋白质互作预测等。结果表明，蓝莓基因组中鉴定出229个非冗余MYB序列，分为23个小组。共鉴定出102个对干旱胁迫有显著响应的MYB DEG，其中叶中72个，根中69个，以及8个表达水平变化> 20倍的差异表达基因。17个DEG与MYB其它成员的表达相关性较高。通过STRING分析结合生理和形态学观察预测了关键VcMYB蛋白的相互作用伴侣。预测VcMYB的10个关键基因如VcMYB 8、VcMYB 102和VcMYB 228可能参与活性氧（ROS）途径，而VcMYB的7个关键基因如VcMYB 41、VcMYB 88和VcMYB 100等可能参与ROS途径。可能参与了干旱处理下叶片的调节。本研究为深入了解VcMYB家族在蓝莓干旱胁迫响应中的调控机制提供了新的思路，为今后在干旱胁迫条件下研究蓝莓的抗旱机理奠定了基础。在线版本包含补充材料，可通过10.1186/s12864-021-07850-5获得。
Blueberry (Vaccinium corymbosum L.) is an important species with a high content of flavonoids in fruits. As a perennial shrub, blueberry is characterized by shallow-rooted property and susceptible to drought stress. MYB transcription factor was reported to be widely involved in plant response to abiotic stresses, however, the role of MYB family in blueberry responding to drought stress remains elusive. In this study, we conducted a comprehensive analysis of VcMYBs in blueberry based on the genome data under drought stress, including phylogenetic relationship, identification of differentially expressed genes (DEGs), expression profiling, conserved motifs, expression correlation and protein-protein interaction prediction, etc. The results showed that 229 non-redundant MYB sequences were identified in the blueberry genome, and divided into 23 subgroups. A total of 102 MYB DEGs with a significant response to drought stress were identified, of which 72 in leaves and 69 in roots, and 8 differential expression genes with a > 20-fold change in the level of expression. 17 DEGs had a higher expression correlation with other MYB members. The interaction partners of the key VcMYB proteins were predicted by STRING analysis and in combination with physiological and morphological observation. 10 key VcMYB genes such as VcMYB8, VcMYB102 and VcMYB228 were predicted to be probably involved in reactive oxygen species (ROS) pathway, and 7 key VcMYB genes (VcMYB41, VcMYB88 and VcMYB100, etc..) probably participated in leaf regulation under drought treatment. Our studies provide a new understanding of the regulation mechanism of VcMYB family in blueberry response to drought stress, and lay fundamental support for future studies on blueberry grown in regions with limited water supply for this crop. The online version contains supplementary material available at 10.1186/s12864-021-07850-5.
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