MiR-29b affects the secretion of PROG and promotes the proliferation of bovine corpus luteum cells.
MiR-29b affects the secretion of PROG and promotes the proliferation of bovine corpus luteum cells.
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MiR-29b影响PROG分泌并促进牛黄体细胞增殖
DOI：
10.1371/journal.pone.0195562
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Zhang JB
中科院分区：
文献类型：
--
作者：
Xu MQ;Jiang H;Zhang LQ;Sun XL;Luo D;Fu Y;Gao Y;Yuan B;Zhang JB
已经探索了miRNAs在卵巢细胞中的调节作用，并且已经揭示了它们对性腺发育、细胞凋亡、排卵、类固醇产生和黄体（CL）发育的影响。本研究分析了miR-29 b在牛CL发育不同阶段的表达情况，并预测了miR-29 b的靶基因。我们证实miR-29 b降低催产素受体（OXTR）的表达，影响孕酮（PROG）分泌并调节CL的功能。RT-PCR结果显示miR-29 b在功能性CL期的表达显著高于消退CL期。免疫组化显示OXTR在大、小CL细胞中均有表达，主要定位于细胞膜和细胞质。我们分析了OXTR的表达水平，发现用miR-29 b模拟物转染降低了OXTR的表达，但用抑制剂转染对OXTR蛋白的表达影响有限。同时，miR-29 b模拟物转染组PROG分泌明显增加。我们还分析了miR-29 b对CL细胞凋亡的影响。最后，我们发现miR-29 b可以促进牛CL细胞的增殖。综上所述，我们发现miR-29 b通过降低OXTR的表达，促进CL细胞的PROG分泌和增殖。
The regulatory role of miRNAs has been explored in ovarian cells, and their effects on gonadal development, apoptosis, ovulation, steroid production and corpus luteum (CL) development have been revealed. In this study, we analyzed the expression of miR-29b at different stages of bovine CL development and predicted the target genes of miR-29b. We confirmed that miR-29b reduces the expression of the oxytocin receptor (OXTR), affects progesterone (PROG) secretion and regulates the function of the CL. RT-PCR showed that the expression of miR-29b was significantly higher in functional CL phases than in the regressed CL phase. Immunohistochemistry showed that OXTR was expressed in both large and small CL cells and was mainly located in the cell membrane and cytoplasm of these cells. We analyzed the expression levels of OXTR and found that transfection with a miR-29b mimic decreased OXTR expression, but transfection with the inhibitor had a limited effect on the expression of the OXTR protein. At the same time, the secretion of PROG was significantly increased in the miR-29b mimic-transfected group. We also analyzed the effect of miR-29b on the apoptosis of CL cells. Finally, we found that miR-29b could promote the proliferation of bovine CL cells. In conclusion, we found that miR-29b reduces the expression of OXTR and can promote PROG secretion and the proliferation of CL cells via OXTR.
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影响因子：
64.5
作者：
Chipuk JE;McStay GP;Bharti A;Kuwana T;Clarke CJ;Siskind LJ;Obeid LM;Green DR
通讯作者：
Green DR
影响因子：
6.3
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影响因子：
8
作者：
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通讯作者：
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影响因子：
3.7
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通讯作者：
Wang Y
影响因子：
2.4
作者：
Ma, Tenghe;Jiang, Hao;Zhang, Jiabao
通讯作者：
Zhang, Jiabao