Partial intercalative binding of the food colorant erythrosine to herring sperm DNA
Partial intercalative binding of the food colorant erythrosine to herring sperm DNA
复制标题
食品着色剂赤藓红与鲱鱼精子 DNA 的部分嵌入结合
DOI：
10.1039/c5ra19626d
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发表时间：
2015-11
期刊：
影响因子：
3.9
通讯作者：
Deming Gong
中科院分区：
文献类型：
--
作者：
Langhong Wang;Mo Tao;Guowen Zhang;Song Li;Deming Gong
红素是一种广泛应用于食品工业的人工色素，但存在潜在的安全风险。本研究通过多光谱技术和对接模拟，确定了鲱鱼和鲱鱼精子DNA （hsDNA）在体外相互作用的特征。采用多元曲线分辨率-交替最小二乘化学计量学方法对扩展后的紫外可见光谱数据矩阵进行处理，同时获得从高度重叠的复合响应中提取的组分（Ery、hsDNA和Ery - hsDNA复合物）的平衡浓度曲线和纯光谱，以监测Ery - hsDNA相互作用过程。基于Ery的低色性和红移效应、更强的双DNA效应和hsDNA粘度的降低，发现Ery与hsDNA结合的主要方式是部分嵌入。傅里叶变换红外光谱和核磁共振光谱显示，Ery的苯环极有可能插入hsDNA的鸟嘌呤和胞嘧啶碱基，分子对接预测并可视化了特异性结合。圆二色性和DNA切割实验表明，高浓度的Ery可能会扰乱B-DNA构象并引起轻微的DNA切割。本研究揭示了Ery与hsDNA的结合机制以及该着色剂的潜在危害。
Erythrosine (Ery) is an artificial colorant extensively used in the food industry, but may have a potential safety risk. In this study, the characteristics of the interaction in vitro between Ery and herring sperm DNA (hsDNA) were determined by multi-spectroscopic techniques and docking simulations. The multivariate curve resolution-alternating least squares chemometrics approach was used to process the expanded UV-vis spectral data matrix, and both the equilibrium concentration profiles and the pure spectra for the components (Ery, hsDNA and Ery–hsDNA complex) extracted from the highly overlapping composite response were obtained simultaneously to monitor the Ery–hsDNA interaction process. Partial intercalation as the dominant mode of Ery binding to hsDNA was found based on the hypochromism and red shift effect of Ery, stronger double DNA effect and decrease in hsDNA viscosity. The Fourier transform infrared and nuclear magnetic resonance spectra showed that the benzene ring of Ery most likely inserted into the guanine and cytosine bases of hsDNA, and molecular docking predicted and visualized the specific binding. The circular dichroism and DNA cleavage assays suggested that Ery at high concentrations may perturb B-DNA conformation and cause slight DNA cleavage. This study has provided insights into the binding mechanism of Ery with hsDNA and potential hazards of the colorant.
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DOI：
10.1016/j.saa.2006.07.027
发表时间：
2007-06
期刊：
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
影响因子：
--
作者：
Feng Wang;Jinghe Yang;Xia Wu;Fei Wang;Honghong Ding
通讯作者：
Feng Wang;Jinghe Yang;Xia Wu;Fei Wang;Honghong Ding
DOI：
10.1016/j.ijbiomac.2015.03.036
发表时间：
2015-07
影响因子：
8.2
作者：
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通讯作者：
A. Subastri;C. Ramamurthy;A. Suyavaran;R. Mareeswaran;P. L. Rao;M. Harikrishna;M. S. Kumar;V. Sujatha
影响因子：
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作者：
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通讯作者：
Tarique Sarwar;M. Husain;S. Rehman;Hassan Mubarak Ishqi;M. Tabish
影响因子：
5.8
作者：
Cao, Jie;Wei, Yin;Cheng, Yan
通讯作者：
Cheng, Yan
影响因子：
3.4
作者：
Gupta, RC;SpencerBeach, G
通讯作者：
SpencerBeach, G