Study on the binding interaction between perfluoroalkyl acids and DNA
Study on the binding interaction between perfluoroalkyl acids and DNA
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全氟烷基酸与DNA结合作用的研究
DOI：
10.1007/s11356-013-1760-4
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发表时间：
2013-05
影响因子：
5.8
通讯作者：
Cheng, Yan
中科院分区：
文献类型：
--
作者：
Cao, Jie;Wei, Yin;Cheng, Yan
全氟烷基酸（PFAA）是致癌物质，阐明其DNA结合特性对于理解PFAA遗传毒性至关重要。利用荧光置换和分子对接技术研究了5种全氟辛酸与7种DNA分子的结合方式和亲和力。通过圆二色性（CD）研究了PFAA结合后DNA构象的变化。数据显示，DNA嵌入是PFAA的主要相互作用模式;然而，这些分子也结合到凹槽。PFAA的解离常数范围为0.11 ~ 1，217.14 μM，DNA嵌入和沟槽结合的解离常数范围为3.46 ~ 2，141.21 μM。含有较长碳链的PFAA具有较强的DNA嵌入亲和力。全氟烷基磺酸盐与DNA的结合比含有相同碳数的全氟羧酸更强。这一观察结果被假定是由于在全氟烷基磺酸盐中存在更多的氟和氧原子作为氢键供体，促进更强的DNA嵌入。PFAA与DNA的结合表现出一定的CT-DNA序列选择性。分子对接分析证实了PFAA的DNA结合模式和亲和力。CD分析表明，PFAA削弱了DNA的碱基堆积，使DNA的螺旋度变松。本研究提高了我们对PFAA-DNA加合物形成的理解。
Perfluoroalkyl acids (PFAAs) are carcinogens, and elucidating their DNA binding properties is crucial for understanding PFAA genotoxicity. We have investigated the binding mode and affinity of five PFAAs to seven DNA molecules using fluorescence displacement and molecular docking analysis. DNA conformational changes upon PFAA binding were also examined by circular dichroism (CD). The data revealed that DNA intercalation was the dominant interaction mode of the PFAAs; however, these molecules also bound to grooves. The dissociation constants for the PFAAs ranged between 0.11 and 1,217.14 μM, and between 3.46 and 2,141.21 μM for DNA intercalation and groove binding, respectively. PFAAs that contain longer carbon chains had stronger DNA intercalation affinities. Binding to DNA was stronger for perfluoroalkyl sulfonates than for perfluorcarboxyl acids that contain the same number of carbons. This observation is postulated to arise from the presence of more fluorine and oxygen atoms in perfluoroalkyl sulfonates acting as hydrogen bond donors that facilitate stronger DNA intercalation. The binding of the PFAAs to DNA showed some CT-DNA sequence selectivity. Molecular docking analysis confirmed the DNA binding mode and affinities of the PFAAs. CD analysis revealed that the PFAAs weakened DNA base stacking and loosened DNA helicity. The present study has improved our understanding of the formation of PFAA–DNA adducts.
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DOI：
10.1016/j.scitotenv.2010.09.024
发表时间：
2010-11
期刊：
The Science of the total environment
影响因子：
--
作者：
Yin Wei;Yuan Lin;Ai-qian Zhang;Liang-Hong Guo;Jie Cao
通讯作者：
Yin Wei;Yuan Lin;Ai-qian Zhang;Liang-Hong Guo;Jie Cao
影响因子：
10.4
作者：
Slotkin TA;MacKillop EA;Melnick RL;Thayer KA;Seidler FJ
通讯作者：
Seidler FJ
影响因子：
64.8
作者：
NELSON, HCM;FINCH, JT;KLUG, A
通讯作者：
KLUG, A
影响因子：
64.8
作者：
ALEXEEV, DG;LIPANOV, AA;SKURATOVSKII, IY
通讯作者：
SKURATOVSKII, IY
影响因子：
5.9
作者：
Vierke, Lena;Staude, Claudia;Schulte, Christoph
通讯作者：
Schulte, Christoph