Entomological signatures in honey: an environmental DNA metabarcoding approach can disclose information on plant-sucking insects in agricultural and forest landscapes.
Entomological signatures in honey: an environmental DNA metabarcoding approach can disclose information on plant-sucking insects in agricultural and forest landscapes.
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DOI：
10.1038/s41598-018-27933-w
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发表时间：
2018-07-03
影响因子：
4.6
通讯作者：
Fontanesi L
中科院分区：
文献类型：
--
作者：
Utzeri VJ;Schiavo G;Ribani A;Tinarelli S;Bertolini F;Bovo S;Fontanesi L
蜜露是由吸食植物的昆虫（半翅目）的排泄物产生的，是一种富含碳水化合物的物质，蜜蜂通过采食来整合其饮食。在这项研究中，我们使用从蜂蜜中提取的 DNA 作为环境 DNA 的来源，揭示了由产生蜜露的半翅目确定的昆虫学特征，这种半翅目不仅从蜜露蜂蜜中回收，而且还从花蜜中回收。我们设计了 PCR 引物，使用从单花、多花和蜜露蜂蜜中分离的 DNA 扩增半翅目物种线粒体细胞色素 c 氧化酶亚基 1 (COI) 基因的片段。 Ion Torrent 下一代测序元条形码数据分析使用定制的生物信息管道分配半翅目物种。森林蜜露蜂蜜报告存在高丰度的梳状 Cinara pectinatae DNA，证实了它们的银杉森林起源。在所有其他蜂蜜中，大多数测序读数来自飞虱 Metcalfa pruinosa，可以评估不同有丝分裂型的频率。从不同地理和植物来源的蜂蜜中鉴定出其他种类的蚜虫。这种独特的昆虫学特征源自蜂蜜中所含的环境 DNA，为蜂蜜认证以及揭示和监测农业和森林景观中植物吸食昆虫的生态开辟了新的应用。
Honeydew produced from the excretion of plant-sucking insects (order Hemiptera) is a carbohydrate-rich material that is foraged by honey bees to integrate their diets. In this study, we used DNA extracted from honey as a source of environmental DNA to disclose its entomological signature determined by honeydew producing Hemiptera that was recovered not only from honeydew honey but also from blossom honey. We designed PCR primers that amplified a fragment of mitochondrial cytochrome c oxidase subunit 1 (COI) gene of Hemiptera species using DNA isolated from unifloral, polyfloral and honeydew honeys. Ion Torrent next generation sequencing metabarcoding data analysis assigned Hemiptera species using a customized bioinformatic pipeline. The forest honeydew honeys reported the presence of high abundance of Cinara pectinatae DNA, confirming their silver fir forest origin. In all other honeys, most of the sequenced reads were from the planthopper Metcalfa pruinosa for which it was possible to evaluate the frequency of different mitotypes. Aphids of other species were identified from honeys of different geographical and botanical origins. This unique entomological signature derived by environmental DNA contained in honey opens new applications for honey authentication and to disclose and monitor the ecology of plant-sucking insects in agricultural and forest landscapes.
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影响因子：
3.7
作者：
Galimberti A;De Mattia F;Bruni I;Scaccabarozzi D;Sandionigi A;Barbuto M;Casiraghi M;Labra M
通讯作者：
Labra M
影响因子：
4.6
作者：
Cheng, H.;Jin, W.;Jia, S.
通讯作者：
Jia, S.
DOI：
10.1073/pnas.1503283112
发表时间：
2015-06-30
影响因子：
11.1
作者：
Kartzinel, Tyler R.;Chen, Patricia A.;Pringle, Robert M.
通讯作者：
Pringle, Robert M.
DOI：
10.1007/s00244-007-9126-x
发表时间：
2008-10-01
影响因子：
4
作者：
Balayiannis, George;Balayiannis, Panos
通讯作者：
Balayiannis, Panos
影响因子：
3.7
作者：
Bertolini F;Ghionda MC;D'Alessandro E;Geraci C;Chiofalo V;Fontanesi L
通讯作者：
Fontanesi L