Development of the petaloid bracts of a paleoherb species, Saururus chinensis.
Development of the petaloid bracts of a paleoherb species, Saururus chinensis.
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古草本植物三白草花瓣状苞片的发育
DOI：
10.1371/journal.pone.0255679
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Li DZ
中科院分区：
文献类型：
--
作者：
Zhao YH;Zhang XM;Li DZ
中国三白草（Saurururus chinensis）是三白草科（Saurururaceae）的核心成员，三白草科是中被子植物门木兰科的一个古老的无花被（无花瓣或萼片）科，其独特的花部组成和花瓣状苞片对了解早期花的起源和进化具有重要意义。为了比较它们的转录组，RNA-seq丰度分析确定了43，463个在S.中国苞片。其中，5，797个表现出显着的差异表达，其中与白色苞片相比，绿色苞片中有1，770个上调，4，027个下调。表达谱也比较了使用cDNA微阵列，其中确定了166个额外的差异表达基因。随后，利用qRT-PCR对cDNA微阵列结果进行验证和扩展，结果表明A类和B类MADS-box基因在白色苞片中表达上调。对推测的S. chinensis A类和B类MADS-box基因的序列分析，以推断MADS-box基因家族A类和B类内的进化关系。此外，B类MADS盒蛋白的自然选择和蛋白质相互作用被推断为B类基因不受进化压力的影响。结果表明，花瓣状苞片显示解剖和基因表达特征通常与花瓣，在其他物种的花瓣状苞片中发现，并支持进化保守的发育程序花瓣状苞片。
Saururus chinensis is a core member of Saururaceae, an ancient, perianthless (lacking petals or sepals) family of the magnoliids in the Mesangiospermae, which is important for understanding the origin and evolution of early flowers due to its unusual floral composition and petaloid bracts. To compare their transcriptomes, RNA-seq abundance analysis identified 43,463 genes that were found to be differentially expressed in S. chinensis bracts. Of these, 5,797 showed significant differential expression, of which 1,770 were up-regulated and 4,027 down-regulated in green compared to white bracts. The expression profiles were also compared using cDNA microarrays, which identified 166 additional differentially expressed genes. Subsequently, qRT-PCR was used to verify and extend the cDNA microarray results, showing that the A and B class MADS-box genes were up-regulated in the white bracts. Phylogenetic analysis was performed on putative S. chinensis A and B-class of MADS-box genes to infer evolutionary relationships within the A and B-class of MADS-box gene family. In addition, nature selection and protein interactions of B class MADS-box proteins were inferred that B-class genes free from evolutionary pressures. The results indicate that petaloid bracts display anatomical and gene expression features normally associated with petals, as found in petaloid bracts of other species, and support an evolutionarily conserved developmental program for petaloid bracts.
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