A multimodal pipeline using NMR spectroscopy and MALDI-TOF mass spectrometry imaging from the same tissue sample.
A multimodal pipeline using NMR spectroscopy and MALDI-TOF mass spectrometry imaging from the same tissue sample.
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作者：
核磁共振波谱和基质辅助激光解吸电离质谱成像 (MALDI MSI) 均常用于在代谢组学和脂质组学研究中检测大量代谢物和脂质。我们展示了一种新的工作流程，强调了这两种技术在获取代谢组学和脂质组学数据方面的优势，首次实现了这两种互补且强大的技术的结合。核磁共振波谱经常用于从细胞和组织中获取定量代谢物信息。核磁共振波谱也可以进行脂质检测，所有类别的分子都可以看到变化。同时，MALDI MSI 提供代谢物和脂质浓度的相对测量，绘制细胞或组织中许多特定代谢物和脂质分子的空间信息。我们结合使用这两种互补技术，从三阴性人类乳腺癌细胞和肿瘤异种移植模型中获得代谢组学和脂质组学测量结果。我们强调了关键的实验程序，以确保在同一样品的组合工作流程中成功实现 NMR 波谱和 MALDI MSI。我们的数据表明，几种磷脂代谢物种类在乳腺肿瘤异种移植物的存活和坏死区域中存在差异性分布。这项研究强调了 NMR 波谱与 MALDI 成像相结合的力量，以推进代谢组学和脂质组学研究。
NMR spectroscopy and matrix assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) are both commonly used to detect large numbers of metabolites and lipids in metabolomic and lipidomic studies. We have demonstrated a new workflow, highlighting the benefits of both techniques to obtain metabolomic and lipidomic data, which has realized for the first time the combination of these two complementary and powerful technologies. NMR spectroscopy is frequently used to obtain quantitative metabolite information from cells and tissues. Lipid detection is also possible with NMR spectroscopy, with changes being visible across entire classes of molecules. Meanwhile, MALDI MSI provides relative measures of metabolite and lipid concentrations, mapping spatial information of many specific metabolite and lipid molecules across cells or tissues. We have used these two complementary techniques in combination to obtain metabolomic and lipidomic measurements from triple-negative human breast cancer cells and tumor xenograft models. We have emphasized critical experimental procedures that ensured the success of achieving NMR spectroscopy and MALDI MSI in a combined workflow from the same sample. Our data show that several phospholipid metabolite species were differentially distributed in viable and necrotic regions of breast tumor xenografts. This study emphasizes the power of combined NMR spectroscopy–MALDI imaging to advance metabolomic and lipidomic studies.
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影响因子：
15.9
作者：
Cheng, LL;Anthony, DC;Gonzalez, RG
通讯作者：
Gonzalez, RG
DOI：
10.1016/j.neo.2017.05.004
发表时间：
2017-08
期刊：
Neoplasia (New York, N.Y.)
影响因子：
--
作者：
Cheng M;Rizwan A;Jiang L;Bhujwalla ZM;Glunde K
通讯作者：
Glunde K
影响因子：
11.2
作者：
Beloueche-Babari, M;Jackson, LE;Ronen, SM
通讯作者：
Ronen, SM
影响因子：
7.4
作者：
Boskamp, Tobias;Lachmund, Delf;Maass, Peter
通讯作者：
Maass, Peter
影响因子：
2.9
作者：
Cao, Maria D.;Doepkens, Mailin;Krishnamachary, Balaji;Vesuna, Farhad;Gadiya, Mayur M.;Lonning, Per E.;Bhujwalla, Zaver M.;Gribbestad, Ingrid S.;Glunde, Kristine
通讯作者：
Glunde, Kristine