Detection and characterization of diverse alpha- and betacoronaviruses from bats in China.
Detection and characterization of diverse alpha- and betacoronaviruses from bats in China.
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从中国蝙蝠的不同α和betacoronavirus的检测和表征。
DOI：
10.1007/s12250-016-3727-3
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发表时间：
2016-02
影响因子：
5.5
通讯作者：
Tu C
中科院分区：
文献类型：
--
作者：
Xu L;Zhang F;Yang W;Jiang T;Lu G;He B;Li X;Hu T;Chen G;Feng Y;Zhang Y;Fan Q;Feng J;Zhang H;Tu C
蝙蝠被认为是α-和β-冠状病毒的重要储存宿主。在这项研究中，不同的冠状病毒（冠状病毒）在951（阳性率为5.3%）蝙蝠肠道标本收集在中国四个省和西藏自治区，通过泛冠状病毒RT-PCR筛选。基于400-nt RNA依赖的RNA聚合酶（RdRP）序列分析，8个属于α冠状病毒属，42个属于β冠状病毒属。在50份阳性标本中，13份扩增出CoV全长RdRP基因，以进一步进行序列比较，其中3个不同的序列（2个来自未报告的省份）进行了全基因组测序。对乙型冠状病毒的两个完整基因组（JTMC 15和JPDB 144）和一个接近完整的乙型冠状病毒基因组（JTAC 2）进行了测序，并预测了它们的基因组结构。目前的研究已经确定了在中国广泛分布的遗传多样性蝙蝠传播冠状病毒的额外数量。通过序列比较和系统发育分析，提出了蝙蝠冠状病毒的两个新种。 可通过10.1007/s12250-016-3727-3获取本文的补充材料，授权用户可访问。
Bats have been implicated as important reservoir hosts of alpha- and betacoronaviruses. In this study, diverse coronaviruses (CoVs) were detected in 50 of 951 (positive rate 5.3%) intestinal specimens of eight bat species collected in four provinces and the Tibet Autonomous Region of China by pan-coronavirus RT-PCR screening. Based on 400-nt RNA-dependent RNA polymerase (RdRP) sequence analysis, eight belonged to genus Alphacoronavirus and 42 to Betacoronavirus. Among the 50 positive specimens, thirteen gave rise to CoV full-length RdRP gene amplification for further sequence comparison, of which three divergent sequences (two from a unreported province) were subjected to full genome sequencing. Two complete genomes of betacoronaviruses (JTMC15 and JPDB144) and one nearly-complete genome of alphacoronavirus (JTAC2) were sequenced and their genomic organization predicted. The present study has identified additional numbers of genetically diverse bat-borne coronaviruses with a wide distribution in China. Two new species of bat CoV, identified through sequence comparison and phylogenetic analysis, are proposed. Supplementary material is available for this article at 10.1007/s12250-016-3727-3 and is accessible for authorized users.
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DOI：
10.1128/genomea.00818-15
发表时间：
2015-08-13
期刊：
Genome announcements
影响因子：
--
作者：
Lu R;Wang Y;Wang W;Nie K;Zhao Y;Su J;Deng Y;Zhou W;Li Y;Wang H;Wang W;Ke C;Ma X;Wu G;Tan W
通讯作者：
Tan W
影响因子：
82.9
作者：
Menachery VD;Yount BL Jr;Debbink K;Agnihothram S;Gralinski LE;Plante JA;Graham RL;Scobey T;Ge XY;Donaldson EF;Randell SH;Lanzavecchia A;Marasco WA;Shi ZL;Baric RS
通讯作者：
Baric RS
影响因子：
2.8
作者：
Moratelli R;Calisher CH
通讯作者：
Calisher CH
影响因子：
7.6
作者：
Báez-Santos YM;St John SE;Mesecar AD
通讯作者：
Mesecar AD
影响因子：
5.4
作者：
Woo, Patrick C. Y.;Wang, Ming;Yuen, Kwok-yung
通讯作者：
Yuen, Kwok-yung