The role of blood in early endometrial–peritoneal interactions in a syngeneic mouse model of endometriosis
The role of blood in early endometrial–peritoneal interactions in a syngeneic mouse model of endometriosis
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血液在同基因小鼠子宫内膜异位症模型中早期子宫内膜-腹膜相互作用中的作用
DOI：
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发表时间：
2011
影响因子：
3.4
通讯作者：
M. Irahara
中科院分区：
文献类型：
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作者：
Ayako Kobayashi;M. Maegawa;S. Yamamoto;Natsuyo Ugumori;Y. Kasai;A. Tani;H. Uemura;A. Kuwahara;T. Matsuzaki;T. Yasui;H. Furumoto;M. Kamada;M. Irahara
目的通过建立免疫功能正常小鼠的同基因移植模型，阐明血液在子宫内膜异位症病变早期发展中的作用。方法腹腔注射子宫内膜碎片加生理盐水或子宫内膜碎片加血诱导C57BL/6小鼠子宫内膜异位症病变。部分子宫内膜碎片加血注射肝素、水蛭素或组织型纤溶酶原激活剂（tPA）。子宫内膜异位症病变在第1、3和5天通过肉眼和显微镜检查进行评估。结果血组子宫内膜异位症病变面积（6.4±1.7 mm2）明显大于生理盐水组（0.5±0.3 mm2）。加入肝素、水蛭素或tPA后，子宫内膜异位症病变的面积明显减少。第1天，6只小鼠中有5只在腹膜上观察到血型子宫内膜异位症病变。子宫内膜异位症病变在第3天和第5天明显大于第1天。第5天，所有小鼠的子宫内膜异位症病变均出现囊性。结论子宫内膜碎片加血可加速子宫内膜异位症病变的早期发展。血液特性可能与早期子宫内膜-腹膜相互作用有关。
PurposeThe aim of this study was to clarify the role of blood in the early stage of development of endometriotic lesions by developing a syngeneic transplantation model using immunocompetent mice.MethodsEndometriotic lesions were induced in C57BL/6 mice by an intraperitoneal injection of endometrial fragments plus saline or endometrial fragments plus blood. Some endometrial fragments plus blood were injected with heparin, hirudin or tissue plasminogen activator (tPA). Endometriotic lesions on days 1, 3 and 5 were evaluated by gross and microscopic findings.ResultsThe areas of endometriotic lesions in the blood group (6.4 ± 1.7 mm2) were significantly larger than those in the saline group (0.5 ± 0.3 mm2). The areas of endometriotic lesions were significantly reduced by the addition of heparin, hirudin or tPA. On day 1, endometriotic lesions in the blood group were observed on the peritoneum in five of the six mice. Endometriotic lesions on days 3 and 5 were significantly larger than those on day 1. On day 5, endometriotic lesions appeared cystic in all the mice.ConclusionsBlood accelerates the early stage of development of endometriotic lesions when endometrial fragments plus blood are injected. Blood property might be involved in early endometrial–peritoneal interactions.
影响因子：
6.7
作者：
Cao, X;Yang, DZ;Parthasarathy, S
通讯作者：
Parthasarathy, S
影响因子：
20.3
作者：
Hu, L;Lee, M;Karpatkin, S
通讯作者：
Karpatkin, S