Amoebal endosymbiont Neochlamydia genome sequence illuminates the bacterial role in the defense of the host amoebae against Legionella pneumophila.
Amoebal endosymbiont Neochlamydia genome sequence illuminates the bacterial role in the defense of the host amoebae against Legionella pneumophila.
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DOI：
10.1371/journal.pone.0095166
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Yamaguchi H
中科院分区：
文献类型：
--
作者：
Ishida K;Sekizuka T;Hayashida K;Matsuo J;Takeuchi F;Kuroda M;Nakamura S;Yamazaki T;Yoshida M;Takahashi K;Nagai H;Sugimoto C;Yamaguchi H
以往的工作表明，专性细胞内阿米巴内共生菌Neochlamydia S13是一株环境衣原体，其阿米巴感染率为100%，但不会引起阿米巴溶解，缺乏对其他宿主阿米巴的可移植性。这些观察到的潜在机制仍不清楚。在本研究中，我们发现宿主阿米巴可以完全逃避军团菌的感染。Neochlamydia S13的基因组序列草案揭示了基本代谢途径的几个缺陷，以及负责蛋白质-蛋白质相互作用的富含亮氨酸重复序列(LRR)和锚蛋白结构域的独特分子。新衣原体S13缺乏完整的三羧酸循环，呼吸链不完整。ADP/ATP转位酶、ATP结合盒转运体和分泌系统(II型和III型)保存完好，但未发现IV型分泌系统。外膜蛋白(OmcB、POMS、76-kDa蛋白和OmpW)数量有限。有趣的是，识别出了预测具有LRRS(30个基因)或ankyrin结构域(1个基因)的独特蛋白质的基因。此外，还发现了33个转座酶，这可能解释了基因组的剧烈修改。综上所述，Neochlamydia S13的基因组特征解释了与宿主阿米巴的亲密相互作用以补偿细菌的代谢缺陷，并阐明了内共生菌在宿主阿米巴防御军团菌感染中的作用。
Previous work has shown that the obligate intracellular amoebal endosymbiont Neochlamydia S13, an environmental chlamydia strain, has an amoebal infection rate of 100%, but does not cause amoebal lysis and lacks transferability to other host amoebae. The underlying mechanism for these observations remains unknown. In this study, we found that the host amoeba could completely evade Legionella infection. The draft genome sequence of Neochlamydia S13 revealed several defects in essential metabolic pathways, as well as unique molecules with leucine-rich repeats (LRRs) and ankyrin domains, responsible for protein-protein interaction. Neochlamydia S13 lacked an intact tricarboxylic acid cycle and had an incomplete respiratory chain. ADP/ATP translocases, ATP-binding cassette transporters, and secretion systems (types II and III) were well conserved, but no type IV secretion system was found. The number of outer membrane proteins (OmcB, PomS, 76-kDa protein, and OmpW) was limited. Interestingly, genes predicting unique proteins with LRRs (30 genes) or ankyrin domains (one gene) were identified. Furthermore, 33 transposases were found, possibly explaining the drastic genome modification. Taken together, the genomic features of Neochlamydia S13 explain the intimate interaction with the host amoeba to compensate for bacterial metabolic defects, and illuminate the role of the endosymbiont in the defense of the host amoebae against Legionella infection.
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