Expression Pattern of G-Protein-Coupled Estrogen Receptor in Myometrium of Uteri with and without Adenomyosis.
Expression Pattern of G-Protein-Coupled Estrogen Receptor in Myometrium of Uteri with and without Adenomyosis.
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伴与不伴子宫腺肌症子宫肌层G蛋白偶联雌激素受体的表达模式
DOI：
10.1155/2017/5974693
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发表时间：
2017
影响因子：
--
通讯作者：
Li TC
中科院分区：
文献类型：
--
作者：
Li JJ;Duan H;Wang S;Sun FQ;Gan L;Tang YQ;Xu Q;Li TC
比较有子宫腺肌病和无子宫腺肌病女性增殖期和分泌期的连接区及子宫肌层外层G蛋白偶联雌激素受体（GPER）的表达。
本研究共纳入76名女性，其中42名患有子宫腺肌病（增殖期，n = 23；分泌期，n = 19），34名作为对照（增殖期，n = 16；分泌期，n = 18）。从连接区（JZ）和子宫肌层外层（OM）提取蛋白质和总RNA。通过蛋白质印迹法和实时定量聚合酶链反应（RT - qPCR）评估GPER蛋白和mRNA的表达水平。
在子宫腺肌病女性中，无论是在连接区还是子宫肌层外层，无论是在增殖期还是分泌期，GPER蛋白和mRNA的表达均显著高于对照组。
与对照组相比，子宫腺肌病中GPER表达显著且持续增加，无论在增殖期还是分泌期，也无论在连接区还是子宫肌层外层，这表明GPER在子宫腺肌病的发病机制中起重要作用。
Objective To compare the expression of G-protein-coupled estrogen receptor (GPER) in the junctional zone and outer myometrium of the proliferative and secretory phases of women with and without adenomyosis. Methods A total of 76 women were included in this study, 42 with adenomyosis (proliferative phase, n = 23; secretory phases, n = 19) and 34 controls (proliferative phase, n = 16; secretory phases, n = 18). Protein and total RNA were extracted from the junctional zone (JZ) and outer myometrium (OM). GPER protein and mRNA expression levels were evaluated by the use of western blotting and real-time quantitative polymerase chain reaction (RT-qPCR). Results The expression of GPER protein and mRNA in women with adenomyosis was significantly higher than that of control subjects, both in the junctional zone and in the outer myometrium and both in the proliferative and in the secretory phases. Conclusion The significant and consistent increase in GPER expression in adenomyosis compared with control subjects, regardless of whether it was in the proliferative or secretory phases and regardless of whether it was in the JZ or OM, suggests that GPER plays an important role in the pathogenesis of the adenomyosis.
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影响因子：
4
作者：
Ignatov T;Modl S;Thulig M;Weißenborn C;Treeck O;Ortmann O;Zenclussen A;Costa SD;Kalinski T;Ignatov A
通讯作者：
Ignatov A
影响因子：
11.2
作者：
Ariazi EA;Brailoiu E;Yerrum S;Shupp HA;Slifker MJ;Cunliffe HE;Black MA;Donato AL;Arterburn JB;Oprea TI;Prossnitz ER;Dun NJ;Jordan VC
通讯作者：
Jordan VC
影响因子：
3.3
作者：
Vrtačnik P;Ostanek B;Mencej-Bedrač S;Marc J
通讯作者：
Marc J
影响因子：
6.7
作者：
Mehasseb, Mohamed Khairy;Bell, Stephen Charles;Habiba, Marwan A.
通讯作者：
Habiba, Marwan A.
影响因子：
8.8
作者：
Krakstad, C.;Trovik, J.;Wik, E.;Engelsen, I. B.;Werner, H. M. J.;Birkeland, E.;Raeder, M. B.;Oyan, A. M.;Stefansson, I. M.;Kalland, K. H.;Akslen, L. A.;Salvesen, H. B.
通讯作者：
Salvesen, H. B.