Genetic analysis of neonatal death with growth retardation in F1 male Dh/+ mice
Genetic analysis of neonatal death with growth retardation in F1 male Dh/+ mice
复制标题
F1代雄性Dh/小鼠生长迟缓新生儿死亡的遗传分析
DOI：
10.1007/s003359901091
复制
发表时间：
1999
期刊：
影响因子：
2.5
通讯作者：
K. Sekikawa
中科院分区：
文献类型：
--
作者：
J. Suto;H. Yamanaka;K. Sekikawa
摘要。在DDD雌性和Dh - Dh/+雄性之间的Dh/+基因型的F1雄性小鼠几乎全部在出生后数天内死亡；然而，在互反交叉中没有观察到这一点。F1Dh/+的男性通常在死亡前表现出生长迟缓。为了确定DDD基因组中可能导致Dh存在异常的遗传位点，我们对（DDD雌性× Dh - +/+雄性）F1雌性× Dh - Dh/+雄性的回交后代进行了连锁分析。从出生之日起检查生长迟缓的外观，并对生长迟缓和正常断奶的Dh/+雄性进行了跨越常染色体和X染色体（Chr）的微卫星标记位点的基因分型。在X染色体远端边缘，靠近DXMit135的微卫星标记处发现了显著的连锁证据。此外，在DDD雌鼠与原株（C57BL/6J、C3H/HeJ和BALB/cA）产生的F1Dh/+雄鼠中，只有♀DDD ×♂（♀DH-Dh /+×♂C3H/HeJ） F1Dh/+雄鼠的后代未表现出致死性和/或生长迟缓。因此，伴有生长迟缓的F1Dh/+雄性致死率是由Dh基因、X Chr和Y Chr相互作用引起的。基于小家鼠Sry基因CAG重复序列长度多态性，C3H/HeJ不同于C57BL/6J、BALB/cA和DH。这些数据表明，在小家鼠中至少存在两种功能类型的Y染色体。
Abstract. Nearly all F1 male mice with Dh/+ genotype between DDD female and DH–Dh/+ male die within a few days after birth; however, this is not observed in the reciprocal cross. The F1Dh/+ males usually exhibit growth retardation prior to death. To identify the putative genetic locus or loci in DDD genome that cause the abnormalities in the presence of the Dh, a linkage analysis was carried out in backcross progeny of a cross of (DDD female × DH–+/+ male) F1 female × DH–Dh/+ male. Appearance of growth retardation was examined from the day of birth, and both growth-retarded and normally weaned Dh/+ males were genotyped for microsatellite marker loci spanning autosomes and the X Chromosome (Chr). Significant evidence for linkage was identified on the distal edge of the X Chr, near the microsatellite marker of DXMit135. Furthermore, among mice from DDD female × reciprocal F1Dh/+ male produced between DH–Dh/+ and progenitor strains (C57BL/6J, C3H/HeJ and BALB/cA), only the progeny from ♀DDD ×♂(♀DH–Dh/+×♂C3H/HeJ) F1Dh/+ male did not show any lethality and/or growth retardation. Thus, the lethality in F1Dh/+ males accompanied by growth retardation is caused by the interactions between the Dh gene, X Chr, and Y Chr. Based on the CAG repeat sequence length polymorphism among Mus musculus musculus Sry gene, C3H/HeJ was different from C57BL/6J, BALB/cA, and DH. These data suggest that there are at least two functional types of Y Chr in Mus musculus musculus.
Sry 等位基因（Mus 亚属）的 DNA 序列分析表明错误调节是 C57BL/6J-Y(POS) 性逆转的原因，并定义了 SRY 功能单元。
影响因子：
3.3
作者：
Albrecht,KH;Eicher,EM
通讯作者：
Eicher,EM
影响因子：
4.4
作者：
Carlisle, C;Winking, H;Nagamine, CM
通讯作者：
Nagamine, CM
小鼠性染色体假常染色体区域高度多态性重复 DNA 序列的结构和染色体定位。
DOI：
10.1159/000132912
发表时间：
1990
期刊：
Cytogenetics and cell genetics
影响因子：
--
作者：
Harbers,K;Francke,U;Soriano,P;Jaenisch,R;Müller,U
通讯作者：
Müller,U