Characterization of the major histocompatibility complex class II DOB, DPB1, and DQB1 alleles in cynomolgus macaques of Vietnamese origin.
Characterization of the major histocompatibility complex class II DOB, DPB1, and DQB1 alleles in cynomolgus macaques of Vietnamese origin.
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DOI：
10.1007/s00251-010-0498-0
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发表时间：
2011-03
期刊：
影响因子：
3.2
通讯作者：
Wang XN
中科院分区：
文献类型：
--
作者：
Ling F;Wei LQ;Wang T;Wang HB;Zhuo M;Du HL;Wang JF;Wang XN
主要组织相容性复合体 (MHC) 分子在许多疾病的易感性和/或抵抗力中发挥着重要作用。为了深入了解 MHC 背景并促进食蟹猴的实验使用，通过克隆测序对 143 只食蟹猴的 MhcMafa-DOB、-DPB1 和 -DQB1 基因的第二外显子进行了表征。总共鉴定出 16 个 Mafa-DOB、16 个 Mafa-DPB1 和 34 个 Mafa-DQB1 等位基因，在越南血统的食蟹猴群体中，DOB、DPB1 和 DQB1 分别存在有限、中等和显着的等位基因多态性。此外，16 个 Mafa-DOB、5 个 Mafa-DPB1 和 8 个 Mafa-DQB1 等位基因代表了早期研究中未报道的新序列。本研究中在 DOB 和 DQB1 位点检测到的几乎所有序列分别属于 DOB*01 (100%) 和 DQB1*06 (62%) 谱系。有趣的是，在这只猴子的 Mafa-DOB、-DPB1 和 -DQB1 上分别检测到了 4 个、3 个和 1 个高频等位基因。这些猴子中频率最高的等位基因是 Mafa-DOB*010102、Mafa-DPB1*13 和 Mafa-DQB1*0616，分别在 129 只猴子中的 33 只（25.6%）、102 只猴子中的 32 只（31.37%）和 143 只猴子中的 30 只（31%）中发现。高频等位基因可能代表免疫功能附加特征的高优先级目标。我们还利用这些序列进行了进化和种群分析，以揭示种群特异性等位基因。这些信息不仅将促进对食蟹猴 MHC 多样性和多态性的理解，还将提高该物种作为生物医学研究模型的价值。
Major histocompatibility complex (MHC) molecules play an important role in the susceptibility and/or resistance to many diseases. To gain an insight into the MHC background and to facilitate the experimental use of cynomolgus macaques, the second exon of the MhcMafa-DOB, -DPB1, and -DQB1 genes from 143 cynomolgus macaques were characterized by cloning to sequencing. A total of 16 Mafa-DOB, 16 Mafa-DPB1, and 34 Mafa-DQB1 alleles were identified, which revealed limited, moderate, and marked allelic polymorphism at DOB, DPB1, and DQB1, respectively, in a cohort of cynomolgus macaques of Vietnamese origin. In addition, 16 Mafa-DOB, 5 Mafa-DPB1, and 8 Mafa-DQB1 alleles represented novel sequences that had not been reported in earlier studies. Almost of the sequences detected at the DOB and DQB1 locus in the present study belonged to DOB*01 (100%) and DQB1*06 (62%) lineages, respectively. Interestingly, four, three, and one high-frequency alleles were detected at Mafa-DOB, -DPB1, and -DQB1, respectively, in this monkeys. The alleles with the highest frequency among these monkeys were Mafa-DOB*010102, Mafa-DPB1*13, and Mafa-DQB1*0616, and these were found in 33 (25.6%) of 129 monkeys, 32 (31.37%) of 102 monkeys, and 30 (31%) of 143 monkeys, respectively. The high-frequency alleles may represent high priority targets for additional characterization of immune function. We also carried out evolutionary and population analyses using these sequences to reveal population-specific alleles. This information will not only promote the understanding of MHC diversity and polymorphism in the cynomolgus macaque but will also increase the value of this species as a model for biomedical research.
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