NIN-like protein 7 transcription factor is a plant nitrate sensor.
NIN-like protein 7 transcription factor is a plant nitrate sensor.
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NIN 样蛋白 7 转录因子是植物硝酸盐传感器
DOI：
10.1126/science.add1104
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发表时间：
2022-09-23
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--
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中科院分区：
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硝酸盐是植物生长所必需的营养物质和信号分子。植物感受胞内硝酸盐以调节其代谢和生长反应。在这里，我们确定了植物中的主要硝酸盐传感器。我们发现，所有七个拟南芥类NIN蛋白（NLP）转录因子的突变废除植物的初级硝酸盐反应和发育程序。NIN-NLP 7嵌合体和硝酸盐结合的分析显示，NLP 7通过其氨基末端在硝酸盐感知时被去阻遏。一种基因编码的荧光分裂生物传感器，mCitrine-NLP 7，实现了植物单细胞硝酸盐动力学的可视化。NLP 7的硝酸盐传感器结构域类似于细菌硝酸盐传感器NreA。配体结合口袋中保守残基的取代损害了硝酸盐触发的NLP 7控制转录、运输、代谢、发育和生物量的能力。我们建议NLP 7代表陆地植物中的硝酸盐传感器。描述转录激活硝酸盐传感器植物依赖于氮，当氮供应变化时，植物的生长和代谢会发生变化。事实上，氮肥是农作物生产力的基础。通过研究一个由七个相似基因组成的家族，刘等人在小型芥菜植物拟南芥中鉴定出了硝酸盐传感器。这种蛋白质的硝酸盐结合口袋类似于细菌硝酸盐传感器。硝酸盐结合后的构象变化允许蛋白质作为转录激活因子发挥作用，触发植物对氮可用性的反应。-PJH一个单一的双功能蛋白质连接硝酸盐传感的变化，在小芥菜植物拟南芥转录。
Nitrate is an essential nutrient and signaling molecule for plant growth. Plants sense intracellular nitrate to adjust their metabolic and growth responses. Here we identify the primary nitrate sensor in plants. We found that mutation of all seven Arabidopsis NIN-like protein (NLP) transcription factors abolished plants’ primary nitrate responses and developmental programs. Analyses of NIN-NLP7 chimeras and nitrate binding revealed that NLP7 is derepressed upon nitrate perception via its amino terminus. A genetically encoded fluorescent split biosensor, mCitrine-NLP7, enabled visualization of single-cell nitrate dynamics in planta. The nitrate sensor domain of NLP7 resembles the bacterial nitrate sensor NreA. Substitutions of conserved residues in the ligand-binding pocket impaired the ability of nitrate-triggered NLP7 to control transcription, transport, metabolism, development, and biomass. We propose that NLP7 represents a nitrate sensor in land plants. Description Transcription-activating nitrate sensor Plants depend on nitrogen, responding with changes in growth and metabolism when nitrogen supplies change. Indeed, nitrogen fertilizer underlies a good deal of agricultural crop productivity. Studying a family of seven similar genes, Liu et al. identified the nitrate sensor in the small mustard plant Arabidopsis thaliana. The protein’s nitrate-binding pocket resembles that found in bacteria nitrate sensors. Conformation change upon nitrate binding allows the protein to then function as a transcriptional activator, triggering the plant’s responses to nitrogen availability. —PJH A single bifunctional protein links nitrate sensing to changes in transcription in the small mustard plant Arabidopsis thaliana.
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影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
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Hassabis D
影响因子：
64.8
作者：
Liu KH;Niu Y;Konishi M;Wu Y;Du H;Sun Chung H;Li L;Boudsocq M;McCormack M;Maekawa S;Ishida T;Zhang C;Shokat K;Yanagisawa S;Sheen J
通讯作者：
Sheen J
影响因子：
16.6
作者：
Marchive, Chloe;Roudier, Francois;Krapp, Anne
通讯作者：
Krapp, Anne
影响因子：
11.6
作者：
Liu, KH;Huang, CY;Tsay, YF
通讯作者：
Tsay, YF
影响因子：
64.8
作者：
Parker, Joanne L.;Newstead, Simon
通讯作者：
Newstead, Simon