Molecular detection of targeted major histocompatibility complex I-bound peptides using a probabilistic measure and nanospray MS3 on a hybrid quadrupole-linear ion trap.
Molecular detection of targeted major histocompatibility complex I-bound peptides using a probabilistic measure and nanospray MS3 on a hybrid quadrupole-linear ion trap.
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DOI：
10.1021/ac102387t
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发表时间：
2010-11-01
影响因子：
7.4
通讯作者：
Reinherz, Ellis L.
中科院分区：
文献类型：
--
作者：
Reinhold, Bruce;Keskin, Derin B.;Reinherz, Ellis L.
基于四极线性离子阱的纳米喷雾MS3方法可以在不分离的情况下从复杂混合物中检测出高动态范围和高灵敏度的目标肽。该方法使用一种改进了相对（Kullback−Leibler， KL）熵表征的概率距离识别算法来检测参考MS3碎片模式是否为获取的MS3光谱的组成部分。该识别反映了物理质谱测量的概率结构，而不像欧几里得或内积度量广泛用于比较光谱。与欧几里得度规或直接相对熵相比，它能够处理具有重要化学离子背景的光谱。完整的纳米喷雾MS3方法允许对目标进行检测和定量，而无需获得同位素标记的标准。通过避免色谱分离及其相关的表面损失，该检测可以应用于非常有限的材料规模的复杂样品。该方法通过应用于检测从有限细胞数量中提取的靶向主要组织相容性复合体（MHC） I相关肽的医学重要问题来说明。
A nanospray MS3 method deployed on a quadrupole linear ion trap hybrid can detect targeted peptides with high dynamic range and high sensitivity from complex mixtures without separations. The method uses a recognition algorithm that is a modification of the relative (Kullback−Leibler, KL) entropy characterization of probabilistic distance to detect if reference MS3 fragmentation patterns are components of acquired MS3 spectra. The recognition reflects the probabilistic structure of physical MS measurements unlike the Euclidean or inner product metrics widely used for comparing spectra. It capably handles spectra with a significant chemical ion background in contrast to the Euclidean metric or the direct relative entropy. The full nanospray MS3 method allows both the detection and quantitation of targets without the need to obtain isotopically labeled standards. By avoiding chromatographic separations and its associated surface losses, the detection can be applied to complex samples on a very limited material scale. The methodology is illustrated by applications to the medically important problem of detecting targeted major histocompatibility complex (MHC) I associated peptides extracted from limited cell numbers.
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影响因子：
2.9
作者：
Bleackley, RC
通讯作者：
Bleackley, RC
影响因子：
3
作者：
Zhang, Zhuo;Sun, Shiwei;Chen, Runsheng
通讯作者：
Chen, Runsheng
影响因子：
7.4
作者：
Fenyö, D;Beavis, RC
通讯作者：
Beavis, RC
DOI：
10.1016/j.jasms.2004.04.034
发表时间：
2004-10-01
影响因子：
3.2
作者：
Tang, KQ;Page, JS;Smith, RD
通讯作者：
Smith, RD
影响因子：
2
作者：
Chernushevich, IV;Bahr, U;Karas, M
通讯作者：
Karas, M