Isolation and characterization of the major histocompatibility complex DQA1 and DQA2 genes in gayal (Bos frontalis)
Isolation and characterization of the major histocompatibility complex DQA1 and DQA2 genes in gayal (Bos frontalis)
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盖亚尔（Bos frontalis）主要组织相容性复合物 DQA1 和 DQA2 基因的分离和表征
DOI：
10.1007/s12041-018-0882-3
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发表时间：
2018-02
影响因子：
1.5
通讯作者：
Xi Dongmei
中科院分区：
文献类型：
--
作者：
Memon Sameeullah;Wang Liping;Li Guozhi;Liu Xiangying;Deng Weidong;Xi Dongmei
云南黄玉的物种起源问题多年来一直存在争议。然而，最近的一些遗传学研究表明，它可能起源于雄性牛与雌性牛或牛之间的杂交。这是一种重要的半野生牛科动物，也被列入国际自然保护联盟的红色名录。然而，关于这种不稳定的Bos物种的免疫原性信息有限。主要组织相容性复合体（MHC）在脊椎动物对传染病的免疫应答中起着关键作用。在本研究中，我们研究了毛蚶（B. frontalis） MHC-DQA基因的结构和功能特征以及可能的重复。扩增MHC-DQA基因的两个全长cDNA克隆，分别命名为bor - dqa1 （DQA*0101）和bor - dqa2 (DQA*2001)， GenBank登录号分别为KT318732和KT318733。将Bofr-DQA1、Bofr-DQA2与来自不同动物物种的其他MHC-DQA分子进行比较，发现这两个MHC-DQA基因的核苷酸和编码氨基酸序列与来自其他反刍动物物种的特定DQA1和DQA2分子等位基因的相似性大于与其他反刍动物物种的特异性DQA1和DQA2分子等位基因的相似性。系统发育研究还表明，这两个基因之间的遗传距离比其他物种的同源基因大。Bofr-DQA1和Bofr-DQA2之间存在较大的遗传距离，并且存在不同的牛DQA推定基序，说明这些序列是非等位基因型。这些结果可能表明DQA基因的重复也发生在绵羊身上。本研究的发现加强了我们对稀有反刍动物MHC多样性以及影响种内和种间MHC变异的免疫功能突变、选择和进化力量的认识。
The species origin of Yunnan gayal has been controversial since many years. However, few recent genetic studies have suggested that it has perhaps originated from the hybridization between male Bos frontalis and female B. taurus or B. indicus. Being an important semi-wild bovid species, this has also been listed under the red list of International Union of Conservation of Nature and Natural Resources. However, there is limited information available about the immunogenicity of this precarious species of Bos. Major histocompatibility complex (MHC) plays a pivotal role in immune response to infectious diseases in vertebrates. In the present study, we have investigated the structural and functional characteristics and possible duplication of the MHC-DQA genes in gayal (B. frontalis). Two full-length cDNA clones of the MHC-DQA genes were amplified and designated as Bofr-DQA1 (DQA*0101) and Bofr-DQA2 (DQA*2001) with GenBank accession numbers KT318732 and KT318733, respectively. A comparison between Bofr-DQA1, Bofr-DQA2 and to other MHC-DQA molecules from different animal species showed that nucleotide and encoded amino acid sequences of these two identified MHC-DQA genes have more similarity to alleles of specific DQA1 and DQA2 molecules from other Ruminantia species than to each other. The phylogenic investigation also demonstrated a large genetic distance between these two genes than to homologous from the other species. The large genetic distance between Bofr-DQA1 and Bofr-DQA2, and the presence of different bovine DQA putative motifs clarify that these sequences are nonallelic type. These results could suggest that duplication of the DQA genes has also occurred in gayal. The findings of the present study have strengthened our understanding to MHC diversity in rare ruminants and mutation of immunological functions, selective and evolutionary forces that affect MHC variation within and between species.
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影响因子：
3.4
作者：
Kuduk K;Babik W;Bojarska K;Sliwińska EB;Kindberg J;Taberlet P;Swenson JE;Radwan J
通讯作者：
Radwan J
DOI：
10.20506/rst.23.3.1523
发表时间：
2004-12
期刊：
Revue scientifique et technique
影响因子：
--
作者：
S. Rajkhowa;C. Rajkhowa;Habibur Rahman;K. Bujarbaruah
通讯作者：
S. Rajkhowa;C. Rajkhowa;Habibur Rahman;K. Bujarbaruah
影响因子：
2.4
作者：
K. Ballingall;B. Marasa;Anthony Luyai;D. McKeever
通讯作者：
K. Ballingall;B. Marasa;Anthony Luyai;D. McKeever
影响因子：
4.4
作者：
Glass, EJ;Oliver, RA;Russell, GC
通讯作者：
Russell, GC
影响因子：
3.2
作者：
S. Sigurdardóttir;C. Borsch;K. Gustafsson;L. Andersson
通讯作者：
S. Sigurdardóttir;C. Borsch;K. Gustafsson;L. Andersson