In vitro and in vivo approaches to study osteocyte biology.
In vitro and in vivo approaches to study osteocyte biology.
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DOI：
10.1016/j.bone.2012.09.040
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发表时间：
2013-06
期刊：
影响因子：
4.1
通讯作者：
Harris, Stephen E.
中科院分区：
文献类型：
--
作者：
Kalajzic, Ivo;Matthews, Brya G.;Torreggiani, Elena;Harris, Marie A.;Pajevic, Paola Divieti;Harris, Stephen E.
骨细胞是骨细胞中数量最多的细胞群，近年来成为骨研究领域的一个热点。这群位于矿化基质中的细胞现在被认为是骨中的机械感觉细胞，在骨形成和吸收的调节中起主要作用。骨细胞的研究受到其位置的影响，导致许多人试图分离原代骨细胞并产生代表骨细胞表型的细胞系。近年来，利用体内遗传学技术，建立了骨细胞定向转基因和基因缺陷小鼠模型，取得了一定的进展。我们将提供一个概述目前在体外和体内模型用于研究骨细胞生物学。我们讨论了骨细胞样细胞系的产生和原代骨细胞的分离，并总结了利用这些细胞模型来了解骨细胞功能作用的研究。将讨论试图选择性地识别和分离骨细胞的方法，使用荧光蛋白报告驱动的基因在骨细胞中高度表达的调控元件。此外，最近在体内的研究，利用过表达或条件删除的各种基因使用牙本质基质蛋白（Dmp 1）指导Cre重组酶进行了概述。总之，评价目前使用的细胞系/遗传模型在理解骨细胞生物学方面的优点和不足，强调了该领域目前的进展。未来的努力将致力于开发新的体外和体内模型，这将有助于理解骨细胞的多种作用。
Osteocytes, the most abundant cell population of the bone lineage, have been a major focus in the bone research field in recent years. This population of cells that resides within mineralized matrix is now thought to be the mechanosensory cell in bone and plays major roles in regulation of bone formation and resorption. Studies of osteocytes had been impaired by their location, resulting in numerous attempts to isolate primary osteocytes and to generate cell lines representative of the osteocytic phenotype. Progress has been achieved in recent years by utilizing in vivo genetic technology and generation of osteocyte directed transgenic and gene deficiency mouse models. We will provide an overview of the current in vitro and in vivo models utilized to study osteocyte biology. We discuss generation of osteocyte-like cell lines and isolation of primary osteocytes and summarize studies that have utilized these cellular models to understand the functional role of osteocytes. Approaches that attempt to selectively identify and isolate osteocytes using fluorescent protein reporters driven by regulatory elements of genes that are highly expressed in osteocytes will be discussed. In addition, recent in vivo studies utilizing overexpression or conditional deletion of various genes using dentin matrix protein (Dmp1) directed Cre recombinase are outlined. In conclusion, evaluation of the benefits and deficiencies of currently used cell lines/genetic models in understanding osteocyte biology underlines the current progress in this field. The future efforts will be directed towards developing novel in vitro and in vivo models that would additionally facilitate understanding the multiple roles of osteocytes.
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影响因子：
4.8
作者：
Divieti, P;Inomata, N;Bringhurst, FR
通讯作者：
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影响因子：
2.3
作者：
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影响因子：
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作者：
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DOI：
10.1016/j.bbrc.2004.06.056
发表时间：
2004-08-06
影响因子：
3.1
作者：
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通讯作者：
Burger, E