Identification of Pseudomonas syringae pv. actinidiae strains causing bacterial canker of kiwifruit in the Anhui Province of China, and determination of their streptomycin sensitivities.
Identification of Pseudomonas syringae pv. actinidiae strains causing bacterial canker of kiwifruit in the Anhui Province of China, and determination of their streptomycin sensitivities.
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丁香假单胞菌 pv. 的鉴定
DOI：
10.4238/2015.july.27.7
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发表时间：
2015-07
期刊：
影响因子：
--
通讯作者：
Xu, Y-L
中科院分区：
文献类型：
--
作者：
Zhang, J-Y;Gao, Z-H;Qi, Y-J;Xu, Y-L
由丁香假单胞菌引起的细菌性口腔溃疡。猕猴桃病是猕猴桃最严重的病害之一。它已成为一种国际流行病，威胁着世界上所有主要猕猴桃种植区猕猴桃生产的可持续发展。链霉素已成为防治猕猴桃溃疡病的主要杀菌剂，特别是在中国猕猴桃主要产区之一的安徽省。然而，到目前为止，还没有关于丁香假单胞菌野外分离株对链霉素基线敏感性的研究。来自中国的actitindiae已经可用。2012-2013年共捕获丁香单菌落102只。从岳西县、金寨县、霍山县、千山县和太湖县分离到放线菌36株、12株、13株、26株和15株。所有菌株经聚合酶链反应扩增后，使用特异性引物PsaF1/R2产生280 bp的片段，然后进行确认致病性的测定，以满足Koch的假设。本研究对102株分离菌株进行了链霉素敏感性测定。链霉素抑制生长的半极大有效浓度为0.03 ~ 0.42 μg/mL（平均0.12±0.06 μg/mL）。基线敏感性曲线为单峰曲线，变异范围因子为14.0。在研究中使用的菌株中未发现耐药亚群。因此，这些敏感性数据可作为监测丁香假单胞菌敏感性变化的基线。安徽省对链霉素的放线菌种群。由于链霉素是一种有风险的杀菌剂，应进行持续耐药性监测。
Bacterial canker, caused by Pseudomonas syringae pv. actinidiae, is one of the most severe diseases of kiwifruit. It has become an international pandemic and threatens the sustainable development of kiwifruit production in all main kiwi-growing regions worldwide. Streptomycin has been the major bactericide for the control of kiwifruit canker, especially in Anhui Province, one of the main kiwifruit production regions in China. However, until now, no studies on the baseline sensitivity to streptomycin of field isolates of P. syringae pv. actinidiae from China have been available. During 2012-2013, a total of 102 single-colony P. syringae pv. actinidiae strains were isolated: 36, 12, 13, 26, and 15 strains from Yuexi, Jinzhai, Huoshan, Qianshan, and Taihu counties, respectively. All strains were confirmed by production of a 280-bp fragment using the specific primers PsaF1/R2 upon polymerase chain reaction amplification, followed by an assay for confirmation of pathogenicity to fulfill Koch's postulates. In this study, the streptomycin sensitivity of the 102 isolated strains was determined. The half-maximal effective concentration values for inhibition of growth by streptomycin were 0.03-0.42 μg/mL (average 0.12 ± 0.06 μg/mL). The baseline sensitivity curve was unimodal, representing range-of-variation factors of 14.0. No resistant subpopulation was identified among the strains used in the study. Thus, these sensitivity data could be used as a baseline for monitoring the shift in sensitivity of P. syringae pv. actinidiae populations to streptomycin in Anhui Province. Continuous resistance monitoring should be carried out, as streptomycin is an at-risk bactericide agent.
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DOI：
--
发表时间：
1994
期刊：
--
影响因子：
--
作者：
Y. Koh;H. Chung;B. Cha
通讯作者：
Y. Koh;H. Chung;B. Cha
影响因子：
2
作者：
Mazzaglia, Angelo;Renzi, Marsilio;Balestra, Giorgio M.
通讯作者：
Balestra, Giorgio M.
影响因子：
2.4
作者：
G. Balestra;A. Mazzaglia;A. Quattrucci;M. Renzi;A. Rossetti
通讯作者：
G. Balestra;A. Mazzaglia;A. Quattrucci;M. Renzi;A. Rossetti
影响因子：
4.5
作者：
T. Popović;Ž. Ivanović;M. Ignjatov;D. Milošević
通讯作者：
T. Popović;Ž. Ivanović;M. Ignjatov;D. Milošević
DOI：
--
发表时间：
1989
期刊：
--
影响因子：
--
作者：
瀧川 雄一;芹沢 拙夫;市川 健;露無 慎二;後藤 正夫
通讯作者：
瀧川 雄一;芹沢 拙夫;市川 健;露無 慎二;後藤 正夫