Loss of metastatic responsiveness to cell shape modulation in a newly characterized B16 melanoma adhesive cell variant.
Loss of metastatic responsiveness to cell shape modulation in a newly characterized B16 melanoma adhesive cell variant.
复制标题
新鉴定的 B16 黑色素瘤粘附细胞变体对细胞形状调节的转移反应性丧失。
作者：
I. Nabi;A. Raz
从在聚（甲基丙烯酸羟乙酯）[聚（HEMA）]包被的平板上悬浮培养生长的B16-F1黑色素瘤细胞中重复选择粘附亚群，导致分离出命名为B16-A10的粘附变体。与B16-F1细胞相比，B16-A10细胞对聚（甲基丙烯酸羟乙酯）包被的板的粘附性更强，并且表达高度粘附的低转移细胞的有组织的肌动蛋白结构特征，这与B16-F1细胞的差的细胞骨架组织相反。在悬浮液中生长时，B16-A10细胞不获得B16-F1细胞的增强的转移能力特征，并且它们表达类似的肺定殖能力，而与培养条件无关。悬浮培养中B16-F1细胞的转移能力增加以前与表面蛋白质对乳过氧化物酶催化的碘化的可及性降低以及这些蛋白质上唾液酸化花生凝集素结合寡糖的表达增加有关。没有显示细胞形状诱导的转移能力增加的B16-A10细胞在悬浮培养中不经历这两种性质中的任何一种的改变。在悬浮培养的B16-A10细胞上不存在这两种现象，表明它们是相互关联的，并参与悬浮培养的B16-F1细胞的转移能力增加。
Repeated selection of an adherent subpopulation from B16-F1 melanoma cells growing in suspension culture on poly(hydroxyethylmethacrylate) [poly(HEMA)] coated plates resulted in the isolation of an adherent variant designated B16-A10. B16-A10 cells are more adherent to poly(hydroxyethylmethacrylate) coated plates than are B16-F1 cells and express an organized actin structure characteristic of highly adherent low metastatic cells as opposed to the poor cytoskeletal organization of B16-F1 cells. Upon growth in suspension, B16-A10 cells do not acquire the enhanced metastatic capability characteristic of B16-F1 cells and they express similar lung colonizing ability irrespective of the culture conditions. The increased metastatic ability of B16-F1 cells in suspension culture has previously been associated with the decreased accessibility of surface proteins to lactoperoxidase catalyzed iodination and with the increased expression of sialylated peanut agglutinin-binding oligosaccharides on these proteins. B16-A10 cells which show no cell shape induced increase in metastatic ability do not undergo alteration in either of these two properties in suspension culture. The absence of these two phenomena on B16-A10 cells grown in suspension indicates that they are interrelated and involved in the increased metastatic ability of B16-F1 cells grown in suspension.
在均匀或梯度电场中进行蛋白质印迹。
影响因子：
2.9
作者：
Gershoni,JM;Davis,FE;Palade,GE
通讯作者：
Palade,GE