Role of CD11/CD18 in shear rate-dependent leukocyte-endothelial cell interactions in cat mesenteric venules.
Role of CD11/CD18 in shear rate-dependent leukocyte-endothelial cell interactions in cat mesenteric venules.
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CD11/CD18 在猫肠系膜小静脉中剪切率依赖性白细胞-内皮细胞相互作用中的作用。
DOI：
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发表时间：
1991
影响因子：
15.9
通讯作者：
D N Granger
中科院分区：
文献类型：
--
作者：
M. Perry;D N Granger
在体内显微镜被用来评估剪切速率（和剪切应力），白细胞滚动速度，白细胞粘附在猫肠系膜制备之间的关系。直径为25 - 35微米的单个小静脉和小动脉中的剪切速率通过动脉环的分级闭塞在宽范围内变化。随着红细胞速度（Vrbc）的降低，白细胞滚动速度（Vwbc）呈线性下降。尽管剪切应力从5 - 25达因/厘米2变化，Vwbc/Vrbc的比率保持恒定。剪切应力的降低与白细胞粘附的增加相关，特别是当Vwbc降低到低于50微米/秒时。在小动脉中，壁剪切速率降低到低于500 s-1时，每100微米长的血管中粘附1 - 3个白细胞，而暴露于相同剪切速率的小静脉中粘附5 - 16个白细胞。在小动脉中，仅在低剪切率下观察到白细胞滚动。在小于250 s-1的剪切速率下，白细胞滚动速度在小动脉中比在小静脉中快，并且小动脉的Vwbc/Vrbc比率为0.08 +/-0.02，这比在类似剪切速率下在小静脉中获得的比率高四倍。在红细胞速度低于2，000微米/秒时，用CD18特异性抗体（mAb）IB4预处理使小静脉中的白细胞滚动速度增加约20微米/秒。mAb IB4在很大程度上阻止了白细胞粘附于小动脉和小静脉，并增加了低剪切下在小静脉中观察到的Vwbc/Vrbc比率，引起白细胞和微血管内皮之间的CD 18依赖性粘附相互作用，并且剪切速率的差异不能解释白细胞在小静脉中比在小动脉中滚动和粘附的更大倾向。
In vivo microscopy was used to assess the relationships among shear rate (and shear stress), leukocyte rolling velocity, and leukocyte adherence in a cat mesentery preparation. Shear rate in individual venules and arterioles of 25-35 microns diameter were varied over a wide range by graded occlusion of an arterial loop. There was a linear decline in leukocyte rolling velocity (Vwbc) as red cell velocity (Vrbc) was reduced. The ratio Vwbc/Vrbc remained constant despite variations in shear stress from 5-25 dyn/cm2. A reduction in shear stress was associated with an increased leukocyte adherence, particularly when Vwbc was reduced below 50 microns/s. Reduction in wall shear rate below 500 s-1 in arterioles allowed 1-3 leukocytes to adhere per 100 microns length of vessel, while venules exposed to the same shear rates had 5-16 adherent leukocytes. In arterioles, leukocyte rolling was only observed at low shear rates. At shear rates less than 250 s-1 leukocyte rolling velocity was faster in arterioles than venules, and the ratio Vwbc/Vrbc for arterioles was 0.08 +/- 0.02, which was fourfold higher than the ratio obtained in venules at similar shear rates. Pretreatment with the CD18-specific antibody (mAb) IB4 increased leukocyte rolling velocity in venules by approximately 20 microns/s at red cell velocities below 2,000 microns/s. mAb IB4 largely prevented the leukocyte adherence to arterioles and venules, and increased the ratio Vwbc/Vrbc observed in venules at low shear elicit a CD18-dependent adhesive interaction between leukocytes and microvascular endothelium, and that differences in shear rates cannot explain the greater propensity for leukocyte rolling and adhesion in venules than arterioles.
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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