Crystal structure of the Alcanivorax borkumensis YdaH transporter reveals an unusual topology.
Crystal structure of the Alcanivorax borkumensis YdaH transporter reveals an unusual topology.
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DOI：
10.1038/ncomms7874
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发表时间：
2015-04-20
影响因子：
16.6
通讯作者：
Yu EW
中科院分区：
文献类型：
--
作者：
Bolla JR;Su CC;Delmar JA;Radhakrishnan A;Kumar N;Chou TH;Long F;Rajashankar KR;Yu EW
The potential of the folic acid biosynthesis pathway as a target for the development of antibiotics has been clinically validated. However, many pathogens have developed resistance to these antibiotics, prompting a reevaluation of potential drug targets within the pathway. The ydaH gene of Alcanivorax borkumensis encodes an integral membrane protein of the AbgT family of transporters for which no structural information was available. Here, we report the crystal structure of A. borkumensis YdaH, revealing a dimeric molecule with an architecture distinct from other families of transporters. YdaH is a bowl-shaped dimer with a solvent-filled basin extending from the cytoplasm to halfway across the membrane bilayer. Each subunit of the transporter contains nine transmembrane helices and two hairpins that suggest a plausible pathway for substrate transport. Further analyses also suggest that YdaH could act as an antibiotic efflux pump and mediate bacterial resistance to sulfonamide antimetabolite drugs.
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影响因子：
3.2
作者：
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通讯作者：
Green, Jacalyn M.
影响因子：
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作者：
Kulathila R;Kulathila R;Indic M;van den Berg B
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影响因子：
16.6
作者：
Ethayathulla, Abdul S.;Yousef, Mohammad S.;Amin, Anowarul;Leblanc, Gerard;Kaback, H. Ronald;Guan, Lan
通讯作者：
Guan, Lan
影响因子：
9.6
作者：
Hyde, John E.;Dittrich, Sabine;Wang, Ping;Sims, Paul F. G.;de Crecy-Lagard, Valrie;Hanson, Andrew D.
通讯作者：
Hanson, Andrew D.
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K