Fabrication of chitosan/silk fibroin composite nanofibers for wound-dressing applications.
Fabrication of chitosan/silk fibroin composite nanofibers for wound-dressing applications.
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DOI：
10.3390/ijms11093529
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发表时间：
2010-09-21
影响因子：
5.6
通讯作者：
Wang HS
中科院分区：
文献类型：
--
作者：
Cai ZX;Mo XM;Zhang KH;Fan LP;Yin AL;He CL;Wang HS
壳聚糖是一种来源丰富的天然多糖，由于其独特的生物相容性、良好的生物降解性和优异的抗菌性能，已被广泛用于各种生物医学应用，特别是作为伤口敷料。本文采用静电纺丝技术制备了壳聚糖/丝素复合纳米纤维膜。用扫描电镜（SEM）观察了共混纳米纤维的形貌，纤维直径随着壳聚糖含量的增加而减小。力学性能测试表明，丝素蛋白的加入提高了CS/SF纳米纤维的力学性能。采用浊度法测定了复合纳米纤维对大肠杆菌（革兰氏阴性菌）和金黄色葡萄球菌（革兰氏阳性菌）的抗菌活性，结果表明复合纳米纤维的抗菌效果因细菌种类而异。此外，通过苏木精-伊红（H&E）染色和MTT法研究了纳米纤维膜对小鼠成纤维细胞的生物相容性，发现纳米纤维膜能促进细胞的粘附和增殖。这些结果表明，所制备的壳聚糖/丝素蛋白（CS/SF）复合纳米纤维膜可能是一个有前途的候选人的伤口愈合应用。
Chitosan, a naturally occurring polysaccharide with abundant resources, has been extensively exploited for various biomedical applications, typically as wound dressings owing to its unique biocompatibility, good biodegradability and excellent antibacterial properties. In this work, composite nanofibrous membranes of chitosan (CS) and silk fibroin (SF) were successfully fabricated by electrospinning. The morphology of electrospun blend nanofibers was observed by scanning electron microscopy (SEM) and the fiber diameters decreased with the increasing percentage of chitosan. Further, the mechanical test illustrated that the addition of silk fibroin enhanced the mechanical properties of CS/SF nanofibers. The antibacterial activities against Escherichia coli (Gram negative) and Staphylococcus aureus (Gram positive) were evaluated by the turbidity measurement method; and results suggest that the antibacterial effect of composite nanofibers varied on the type of bacteria. Furthermore, the biocompatibility of murine fibroblast on as-prepared nanofibrous membranes was investigated by hematoxylin and eosin (H&E) staining and MTT assays in vitro, and the membranes were found to promote the cell attachment and proliferation. These results suggest that as-prepared chitosan/silk fibroin (CS/SF) composite nanofibrous membranes could be a promising candidate for wound healing applications.
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影响因子：
3.2
作者：
SRINIVASAN, G;RENEKER, DH
通讯作者：
RENEKER, DH
影响因子：
3.2
作者：
Yarin, AL;Koombhongse, S;Reneker, DH
通讯作者：
Reneker, DH
影响因子：
3.5
作者：
Reneker, DH;Chun, I
通讯作者：
Chun, I
DOI：
10.1016/s0965-9773(97)00036-6
发表时间：
1997-01-01
期刊：
NANOSTRUCTURED MATERIALS
影响因子：
--
作者：
Hulteen, JC;Chen, HX;Martin, CR
通讯作者：
Martin, CR
影响因子：
9.7
作者：
Chen, Z. G.;Wang, P. W.;Cui, F. Z.
通讯作者：
Cui, F. Z.