Single Cell Analysis Linking Ribosomal (r)DNA and rRNA Copy Numbers to Cell Size and Growth Rate Provides Insights into Molecular Protistan Ecology.
Single Cell Analysis Linking Ribosomal (r)DNA and rRNA Copy Numbers to Cell Size and Growth Rate Provides Insights into Molecular Protistan Ecology.
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DOI：
10.1111/jeu.12425
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发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Gong J
中科院分区：
文献类型：
--
作者：
Fu R;Gong J
核糖体（r）RNA和rDNA已成为微生物生态学研究的黄金分子标记。然而，它仍然知之甚少核糖体拷贝数（CN）为基础的特征是如何与多样性，丰度和活动的原生生物种群和群落在生物体水平上观察。在这里，我们应用单细胞方法来量化在不同温度下饲养的两种纤毛虫物种中的核糖型CN。我们发现，在活跃生长的细胞中，每个细胞的rDNA和rRNA CN与细胞体积（CV）的比例分别为0.44和0.58。因此，模拟的rDNA和rRNA浓度似乎在较小的细胞中比在较大的细胞中高得多。观察到的rRNA：rDNA比的CV为0.14。最大生长速率可以通过每细胞核糖核酸型CN和温度的组合来很好地预测。我们的经验数据和单细胞核糖体型缩放模型与生态学的代谢理论和生长速率假设一致，为将细胞rDNA和rRNA CN与身体大小，生长（活动）和生物量化学计量联系起来提供了定量框架。这项研究还表明，rRNA基因的表达率受到细胞大小的限制，有利于生物量，而不是基于丰度的解释数量核糖体型数据在种群和群落生态的原生生物。
Ribosomal (r)RNA and rDNA have been golden molecular markers in microbial ecology. However, it remains poorly understood how ribotype copy number (CN)‐based characteristics are linked with diversity, abundance, and activity of protist populations and communities observed at organismal levels. Here, we applied a single‐cell approach to quantify ribotype CNs in two ciliate species reared at different temperatures. We found that in actively growing cells, the per‐cell rDNA and rRNA CNs scaled with cell volume (CV) to 0.44 and 0.58 powers, respectively. The modeled rDNA and rRNA concentrations thus appear to be much higher in smaller than in larger cells. The observed rRNA:rDNA ratio scaled with CV 0.14. The maximum growth rate could be well predicted by a combination of per‐cell ribotype CN and temperature. Our empirical data and modeling on single‐cell ribotype scaling are in agreement with both the metabolic theory of ecology and the growth rate hypothesis, providing a quantitative framework for linking cellular rDNA and rRNA CNs with body size, growth (activity), and biomass stoichiometry. This study also demonstrates that the expression rate of rRNA genes is constrained by cell size, and favors biomass rather than abundance‐based interpretation of quantitative ribotype data in population and community ecology of protists.
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影响因子：
2.9
作者：
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DOI：
10.1073/pnas.0504756102
发表时间：
2005-08-16
影响因子：
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