Transcriptomic and epigenomic remodeling occurs during vascular cambium periodicity in Populus tomentosa.
Transcriptomic and epigenomic remodeling occurs during vascular cambium periodicity in Populus tomentosa.
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毛白杨维管形成层周期性期间发生转录组和表观基因组重塑
DOI：
10.1038/s41438-021-00535-w
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发表时间：
2021-05-01
影响因子：
8.7
通讯作者：
Li R
中科院分区：
文献类型：
--
作者：
Chen B;Xu H;Guo Y;Grünhofer P;Schreiber L;Lin J;Li R
Trees in temperate regions exhibit evident seasonal patterns, which play vital roles in their growth and development. The activity of cambial stem cells is the basis for regulating the quantity and quality of wood, which has received considerable attention. However, the underlying mechanisms of these processes have not been fully elucidated. Here we performed a comprehensive analysis of morphological observations, transcriptome profiles, the DNA methylome, and miRNAs of the cambium in Populus tomentosa during the transition from dormancy to activation. Anatomical analysis showed that the active cambial zone exhibited a significant increase in the width and number of cell layers compared with those of the dormant and reactivating cambium. Furthermore, we found that differentially expressed genes associated with vascular development were mainly involved in plant hormone signal transduction, cell division and expansion, and cell wall biosynthesis. In addition, we identified 235 known miRNAs and 125 novel miRNAs. Differentially expressed miRNAs and target genes showed stronger negative correlations than other miRNA/target pairs. Moreover, global methylation and transcription analysis revealed that CG gene body methylation was positively correlated with gene expression, whereas CHG exhibited the opposite trend in the downstream region. Most importantly, we observed that the number of CHH differentially methylated region (DMR) changes was the greatest during cambium periodicity. Intriguingly, the genes with hypomethylated CHH DMRs in the promoter were involved in plant hormone signal transduction, phenylpropanoid biosynthesis, and plant–pathogen interactions during vascular cambium development. These findings improve our systems-level understanding of the epigenomic diversity that exists in the annual growth cycle of trees.
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DOI：
10.1038/nrm4043
发表时间：
2015-09
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Du J;Johnson LM;Jacobsen SE;Patel DJ
通讯作者：
Patel DJ
植物铬甲基转移酶的DNA甲基化特征。
影响因子：
4.5
作者：
Gouil Q;Baulcombe DC
通讯作者：
Baulcombe DC
影响因子：
7
作者：
Gent JI;Ellis NA;Guo L;Harkess AE;Yao Y;Zhang X;Dawe RK
通讯作者：
Dawe RK
比较 WGBS 鉴定了影响番茄表突变体中不成熟表型的基因 无色不成熟
影响因子：
9.1
作者：
Chen, Weiwei;Yu, Zhiming;Hong, Yiguo
通讯作者：
Hong, Yiguo
DOI：
10.1126/science.1224839
发表时间：
2012-09-14
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ibarra CA;Feng X;Schoft VK;Hsieh TF;Uzawa R;Rodrigues JA;Zemach A;Chumak N;Machlicova A;Nishimura T;Rojas D;Fischer RL;Tamaru H;Zilberman D
通讯作者：
Zilberman D