The SpoVA membrane complex is required for dipicolinic acid import during sporulation and export during germination.
The SpoVA membrane complex is required for dipicolinic acid import during sporulation and export during germination.
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DOI：
10.1101/gad.349488.122
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发表时间：
2022-05-01
影响因子：
10.5
通讯作者：
Rudner, David Z.
中科院分区：
文献类型：
--
作者：
Gao, Yongqiang;Barajas-Ornelas, Rocio Del Carmen;Amon, Jeremy D.;Ramirez-Guadiana, Fernando H.;Alon, Assaf;Brock, Kelly P.;Marks, Debora S.;Kruse, Andrew C.;Rudner, David Z.
在这里，高等人。研究了枯草芽孢杆菌 spoVA 操纵子中编码的小分子吡啶二羧酸（DPA）输入所需的最小蛋白质组，该蛋白质在休眠孢子的抗逆性和萌发过程中退出休眠方面发挥着核心作用，并证明这些蛋白质形成膜复合物。作者提出了一个模型，其中 DPA 转运到孢子中涉及拔出然后重新插入 C-Eb 膜通道的循环，而发芽过程中的营养物检测则通过拔出通道来触发 DPA 释放。为了应对饥饿，形成内生孢子的细菌分化成抗应激孢子，这些孢子可以保持休眠数年，但会根据营养物质迅速发芽并恢复生长。小分子吡啶二羧酸 (DPA) 在休眠孢子的抗逆性和萌发过程中摆脱休眠方面发挥着核心作用。 spoVA 基因座是孢子形成过程中 DPA 输入所必需的，并且与萌发过程中 DPA 的输出有关，但其分子基础尚不清楚。在这里，我们定义了 DPA 导入所需的枯草芽孢杆菌 spoVA 操纵子中编码的最小蛋白质集，并证明这些蛋白质形成膜复合物。这些成分的结构建模结合诱变和体内分析表明，C 和 Eb 亚基形成膜通道，而 D 亚基则充当细胞质塞。我们发现，损害 D 和 C-Eb 膜复合物之间相互作用的点突变会降低孢子形成过程中 DPA 输入的效率，并反过来加速萌发过程中 DPA 的释放。我们的数据支持一个模型，其中 DPA 运输到孢子中涉及拔出然后重新插入 C-Eb 膜通道的循环，而发芽过程中的营养物检测则通过拔出通道来触发 DPA 释放。
Here, Gao et al. investigated the minimal set of proteins encoded in the Bacillus subtilis spoVA operon required for small molecule dipicolinic acid (DPA) import, which plays a central role in both the stress resistance of the dormant spore and its exit from dormancy during germination, and demonstrate that these proteins form a membrane complex. The authors propose a model in which DPA transport into spores involves cycles of unplugging then replugging the C–Eb membrane channel, while nutrient detection during germination triggers DPA release by unplugging it. In response to starvation, endospore-forming bacteria differentiate into stress-resistant spores that can remain dormant for years yet rapidly germinate and resume growth in response to nutrients. The small molecule dipicolinic acid (DPA) plays a central role in both the stress resistance of the dormant spore and its exit from dormancy during germination. The spoVA locus is required for DPA import during sporulation and has been implicated in its export during germination, but the molecular bases are unclear. Here, we define the minimal set of proteins encoded in the Bacillus subtilis spoVA operon required for DPA import and demonstrate that these proteins form a membrane complex. Structural modeling of these components combined with mutagenesis and in vivo analysis reveal that the C and Eb subunits form a membrane channel, while the D subunit functions as a cytoplasmic plug. We show that point mutations that impair the interactions between D and the C–Eb membrane complex reduce the efficiency of DPA import during sporulation and reciprocally accelerate DPA release during germination. Our data support a model in which DPA transport into spores involves cycles of unplugging and then replugging the C–Eb membrane channel, while nutrient detection during germination triggers DPA release by unplugging it.
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影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
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影响因子：
64.5
作者：
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影响因子：
5.2
作者：
Berendsen EM;Koning RA;Boekhorst J;de Jong A;Kuipers OP;Wells-Bennik MH
通讯作者：
Wells-Bennik MH
影响因子：
3.2
作者：
Amon, Jeremy D.;Artzi, Lior;Rudner, David Z.
通讯作者：
Rudner, David Z.
影响因子：
11
作者：
Berendsen, Erwin M.;Boekhorst, Jos;Wells-Bennik, Marjon H. J.
通讯作者：
Wells-Bennik, Marjon H. J.