Primary versus castration-resistant prostate cancer: modeling through novel murine prostate cancer cell lines.
Primary versus castration-resistant prostate cancer: modeling through novel murine prostate cancer cell lines.
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DOI：
10.18632/oncotarget.8436
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发表时间：
2016-05-17
期刊：
影响因子：
--
通讯作者：
Abou-Kheir W
中科院分区：
文献类型：
--
作者：
Daoud G;Monzer A;Bahmad H;Chamaa F;Hamdar L;Mouhieddine TH;Shayya S;Eid A;Kobeissy F;Liu YN;Abou-Kheir W
代表前列腺癌（PC）从雄激素依赖性发展到雄激素非依赖性状态的细胞系很少。在这项研究中，我们使用以前的特点前列腺腔上皮细胞系（梅花），在雄激素的影响下，建立PC进展的细胞模型。已分离源自原位肿瘤的细胞以开发雄激素依赖性（PLum-AD）与雄激素非依赖性（PLum-AI）模型。经免疫荧光、qRT-PCR和Western印迹分析，PLum-AD细胞主要表达前列腺上皮标志物，而PLum-AI细胞表达间充质细胞标志物。有趣的是，两种细胞系都维持了干/祖细胞群体。此外，我们的数据表明，这两种细胞系是致瘤性; PLum-AD导致腺癌，而PLum-AI导致肉瘤样癌时，皮下移植在NOD-SCID小鼠。最后，基因表达谱显示富集的功能参与细胞迁移，凋亡，以及肿瘤的侵袭和转移PLum-AI细胞。总之，这些数据表明，新分离的细胞系代表了一种新的雄激素依赖性和非依赖性PC的体外模型。
Cell lines representing the progression of prostate cancer (PC) from an androgen-dependent to an androgen-independent state are scarce. In this study, we used previously characterized prostate luminal epithelial cell line (Plum), under androgen influence, to establish cellular models of PC progression. Cells derived from orthotopic tumors have been isolated to develop an androgen-dependent (PLum-AD) versus an androgen-independent (PLum-AI) model. Upon immunofluorescent, qRT-PCR and Western blot analyses, PLum-AD cells mostly expressed prostate epithelial markers while PLum-AI cells expressed mesenchymal cell markers. Interestingly, both cell lines maintained a population of stem/progenitor cells. Furthermore, our data suggest that both cell lines are tumorigenic; PLum-AD resulted in an adenocarcinoma whereas PLum-AI resulted in a sarcomatoid carcinoma when transplanted subcutaneously in NOD-SCID mice. Finally, gene expression profiles showed enrichment in functions involved in cell migration, apoptosis, as well as neoplasm invasiveness and metastasis in PLum-AI cells. In conclusion, these data suggest that the newly isolated cell lines represent a new in vitro model of androgen-dependent and –independent PC.
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影响因子：
2.3
作者：
Son H;Moon A
通讯作者：
Moon A
影响因子：
3.4
作者：
Spreafico, Anna;Chi, Kim N.;Sridhar, Srikala S.;Smith, David C.;Carducci, Michael A.;Kavsak, Peter;Wong, Tracy S.;Wang, Lisa;Ivy, S. Percy;Mukherjee, Som Dave;Kollmannsberger, Christian K.;Sukhai, Mahadeo A.;Takebe, Naoko;Kamel-Reid, Suzanne;Siu, Lillian L.;Hotte, Sebastien J.
通讯作者：
Hotte, Sebastien J.
影响因子：
11.2
作者：
Mulholland DJ;Xin L;Morim A;Lawson D;Witte O;Wu H
通讯作者：
Wu H
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
2.1
作者：
Irizarry, RA;Hobbs, B;Speed, TP
通讯作者：
Speed, TP