Importance of compartment formation for a self-encoding system
Importance of compartment formation for a self-encoding system
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区室形成对于自编码系统的重要性
DOI：
--
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发表时间：
2002
影响因子：
11.1
通讯作者：
T. Yomo
中科院分区：
文献类型：
--
作者：
Tomoaki Matsuura;Muneyoshi Yamaguchi;Elizabeth P. Ko;Y. Shima;I. Urabe;T. Yomo
建立了一种自编码系统，其设计具有严格的分子“区隔”，即每个区室中仅包含一个 DNA 分子，并分析了其进化命运。该系统包括作为信息分子的嗜热栖热菌 DNA 聚合酶基因和作为功能分子的复制该基因的蛋白质产物。施加严格的分区允许自编码系统至少持续到第十代，而当施加从100个分子开始的松散分区时，该系统在第三代之后停止工作。这些结果提供了实验证据，证明了划分对于维护自编码系统的重要性。此外，人们发现区室自我复制活动的多样性程度是严格区室和松散区室之间进化动力学的另一个重要差异。尽管严格划分的系统在每一代都提供了广泛多样化的隔室活性，但在松散划分的系统中，活性值仅在很小的范围内发散。当区室活动的多样性较小时，功能选择就会变弱，符合达尔文进化论可能变得不可行。因此，严格的划分对于自编码系统的可进化性至关重要。
A self-encoding system designed to have strict “compartition” of the molecules, i.e., to contain only a single molecule of DNA in each compartment, was established, and its evolutionary fate was analyzed. The system comprised the Thermus thermophilus DNA polymerase gene as the informational molecule and its protein product replicating the gene as the functional molecule. Imposing strict compartition allows the self-encoding system to last up to at least the tenth generation, whereas the system ceased to work after the third generation when loose compartition initiated with 100 molecules was imposed. These results provide experimental evidence on the importance of compartition for the maintenance of a self-encoding system. In addition, the extent of diversity in self-replication activity of the compartments was found to be another vital difference in the evolutionary dynamics between the strict and loose compartitions. Although the system with strict compartition provides widely diversified activity of the compartments at each generation, the values of the activity diverge only within a small range in the system with loose compartition. When the variety in the activity of a compartment is small, functional selection becomes weak, and to conform Darwinian evolution may become unfeasible. Therefore, strict compartition is essential for the evolvability of a self-encoding system.
DOI：
10.1073/pnas.91.13.6093
发表时间：
1994-06-21
影响因子：
11.1
作者：
BREAKER, RR;JOYCE, GF
通讯作者：
JOYCE, GF
影响因子：
56.9
作者：
INOUE, T;ORGEL, LE
通讯作者：
ORGEL, LE