Central and peripheral α-synuclein in Parkinson disease detected by seed amplification assay.
Central and peripheral α-synuclein in Parkinson disease detected by seed amplification assay.
复制标题
通过种子扩增测定检测帕金森病的中央和外周 α-突触核蛋白。
DOI：
10.1002/acn3.51753
复制
发表时间：
2023-05
影响因子：
5.3
通讯作者：
Syst Synuclein Sampling Study
中科院分区：
文献类型：
--
作者：
Chahine, Lana G.;Beach, Thomas H.;Adler, Charles;Hepker, Monica;Kanthasamy, Anumantha;Appel, Scott;Pritzkow, Sandra;Pinho, Michelle;Mosovsky, Sherri E.;Serrano, Geidy;Coffey, Christopher C.;Brumm, Michael;Oliveira, Luis M. A.;Eberling, Jamie;Mollenhauer, Brit;Syst Synuclein Sampling Study
通过种子扩增检测α‐突触核蛋白聚集体是一种很有前途的帕金森病生物标志物检测方法。了解α -突触核蛋白测量的个体内部关系可以为最佳生物标志物的开发提供信息。目的是测试中央（脑脊液）和外周（颌下腺）来源α‐synuclein种子扩增法的准确性，与总α‐synuclein测量值进行比较，并调查受试者之间的关系。系统性突触核蛋白取样研究旨在表征帕金森病患者（n = 59）的多种组织和生物体液中的α‐突触核蛋白，并与健康对照组（n = 21）进行比较。获得运动和非运动测量以及多巴胺转运体扫描。我们比较了四种α - synuclein的测定方法：脑脊液和福尔马林固定石蜡包埋的下颌骨腺种子扩增法，酶联免疫法定量生物体液中α - synuclein总量，免疫组织化学法检测下颌骨腺中α - synuclein聚集量。研究了种子扩增法诊断帕金森病的准确性，并比较了受试者内α -突触核蛋白测定方法。α‐突触核蛋白种子扩增法诊断帕金森病在脑脊液中的敏感性和特异性分别为92.6%和90.5%，在颌下腺中的敏感性和特异性分别为73.2%和78.6%。25/38（65.8%）帕金森病患者脑脊液和颌下腺种子扩增试验均呈阳性。比较不同α‐突触核蛋白检测对帕金森病的诊断准确率，脑脊液种子扩增法最高（约登指数= 83.1%）。98.3%的帕金森病患者α -突触核蛋白≥1项检测阳性。α - synuclein种子扩增试验（脑脊液>颌下腺）与总α - synuclein测量相比具有更高的敏感性和特异性，并且出现了中枢和外周α - synuclein测量在受试者内部的关系。
Detection of α‐synuclein aggregates by seed amplification is a promising Parkinson disease biomarker assay. Understanding intraindividual relationships of α‐synuclein measures could inform optimal biomarker development. The objectives were to test accuracy of α‐synuclein seed amplification assay in central (cerebrospinal fluid) and peripheral (submandibular gland) sources, compare to total α‐synuclein measures, and investigate within‐subject relationships. The Systemic Synuclein Sampling Study aimed to characterize α‐synuclein in multiple tissues and biofluids within Parkinson disease subjects (n = 59) and compared to healthy controls (n = 21). Motor and non‐motor measures and dopamine transporter scans were obtained. Four measures of α‐synuclein were compared: seed amplification assay in cerebrospinal fluid and formalin‐fixed paraffin‐embedded submandibular gland, total α‐synuclein quantified in biofluids using enzyme‐linked immunoassay, and aggregated α‐synuclein in submandibular gland detected by immunohistochemistry. Accuracy of seed amplification assay for Parkinson disease diagnosis was examined and within‐subject α‐synuclein measures were compared. Sensitivity and specificity of α‐synuclein seed amplification assay for Parkinson disease diagnosis was 92.6% and 90.5% in cerebrospinal fluid, and 73.2% and 78.6% in submandibular gland, respectively. 25/38 (65.8%) Parkinson disease participants were positive for both cerebrospinal fluid and submandibular gland seed amplification assay. Comparing accuracy for Parkinson disease diagnosis of different α‐synuclein measures, cerebrospinal fluid seed amplification assay was the highest (Youden Index = 83.1%). 98.3% of all Parkinson disease cases had ≥1 measure of α‐synuclein positive. α‐synuclein seed amplification assay (cerebrospinal fluid>submandibular gland) had higher sensitivity and specificity compared to total α‐synuclein measures, and within‐subject relationships of central and peripheral α‐synuclein measures emerged.
登录
查看更多内容
影响因子：
4.7
作者：
Mollenhauer B;Bowman FD;Drake D;Duong J;Blennow K;El-Agnaf O;Shaw LM;Masucci J;Taylor P;Umek RM;Dunty JM;Smith CL;Stoops E;Vanderstichele H;Schmid AW;Moniatte M;Zhang J;Kruse N;Lashuel HA;Teunissen C;Schubert T;Dave KD;Hutten SJ;Zetterberg H
通讯作者：
Zetterberg H
影响因子：
8.6
作者：
Barba, Lorenzo;Paolini Paoletti, Federico;Bellomo, Giovanni;Gaetani, Lorenzo;Halbgebauer, Steffen;Oeckl, Patrick;Otto, Markus;Parnetti, Lucilla
通讯作者：
Parnetti, Lucilla
影响因子：
2.9
作者：
DOTY, RL;SHAMAN, P;DANN, M
通讯作者：
DANN, M
影响因子：
5.2
作者：
Chahine, Lana M.;Beach, Thomas G.;Mollenhauer, Brit
通讯作者：
Mollenhauer, Brit
影响因子：
8.6
作者：
Kang, Un Jung;Boehme, Amelia K.;Soto, Claudio
通讯作者：
Soto, Claudio