Edinburgh Research Explorer Fine-structure molecular epidemiological analysis of Staphylococcus aureus recovered from cows

Edinburgh Research Explorer Fine-structure molecular epidemiological analysis of Staphylococcus aureus recovered from cows
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爱丁堡研究探索者从奶牛中回收的金黄色葡萄球菌的精细结构分子流行病学分析

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通讯作者:
J. Fitzgerald
J. Fitzgerald
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作者:
J. R. FITZGERALD;W. Meaney;P. Hartigan;C. J. S. and;V. Kapur;J. Fitzgerald

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从美国和爱尔兰共和国的牛源中回收的6 3株金黄色葡萄球菌在两个不同的实验室进行了多位点酶电泳法(MLEE)、核糖体测序法和随机扩增多态性fi聚合酶链式反应分型。S。MLEE将金黄色葡萄球菌分为10个电泳型(区分指数D(CID:175)0(CID:177)779)。与之相反,同一分离株被分成13个核糖体类型(D(Cid:175)0(Cid:177)888)和12个RAPD型(D(Cid:175)0(Cid:177)898)。分布于世界各地的一个常见克隆ET3由核糖型和RAPD型的六种不同组合表示。S。在爱尔兰从奶牛身上发现的金黄色克隆也与美国奶牛的乳房炎有关。这些fi序列与S只有几个特化克隆的假设是一致的。金黄色葡萄球菌是大多数牛乳房炎病例的罪魁祸首,而且这些克隆有广泛的地理分布。
Sixty-three Staphylococcus aureus isolates recovered from bovine sources in the USA and the Republic of Ireland were characterized by multilocus enzyme electrophoresis (MLEE), ribotyping, and random amplified polymorphic DNA polymerase chain reaction (RAPD–PCR) typing at two separate laboratories. The S . aureus isolates were assigned by MLEE to 10 electrophoretic types (ETs) (Index of Discrimination, D (cid:175) 0 (cid:177) 779). In contrast, the same isolates were assigned to 13 ribotypes ( D (cid:175) 0 (cid:177) 888), and to 12 RAPD types ( D (cid:175) 0 (cid:177) 898). A common clone, ET3, of worldwide distribution, was represented by six distinct combinations of ribotypes and RAPD types. S . aureus clones recovered from cows in Ireland were also associated with mastitis in dairy cows in the USA. These findings are consistent with the hypothesis that only a few specialized clones of S . aureus are responsible for the majority of cases of bovine mastitis, and that these clones have a broad geographic distribution.
RAPD(任意引物)PCR 在区分相关细菌菌株方面比多位点酶电泳更灵敏。
DOI: 10.1093/nar/21.25.5930
发表时间: 1993
影响因子: 14.9
作者:
Wang,G;Whittam,TS;Berg,CM;Berg,DE
通讯作者: Berg,DE
DOI: 10.1093/nar/20.19.5137
发表时间: 1992-10-11
影响因子: 14.9
作者:
AKOPYANZ, N;BUKANOV, NO;BERG, DE
通讯作者: BERG, DE
丁香假单胞菌两个致病变型的遗传多样性和关系。
DOI: 10.1099/00221287-134-7-1949
发表时间: 1988
期刊: Journal of general microbiology
影响因子: --
作者:
Denny,TP;Gilmour,MN;Selander,RK
通讯作者: Selander,RK