Antarctic Krill Oil Diet Protects against Lipopolysaccharide-Induced Oxidative Stress, Neuroinflammation and Cognitive Impairment.
Antarctic Krill Oil Diet Protects against Lipopolysaccharide-Induced Oxidative Stress, Neuroinflammation and Cognitive Impairment.
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DOI：
10.3390/ijms18122554
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发表时间：
2017-11-28
影响因子：
5.6
通讯作者：
Hong JT
中科院分区：
文献类型：
--
作者：
Choi JY;Jang JS;Son DJ;Im HS;Kim JY;Park JE;Choi WR;Han SB;Hong JT
氧化应激和神经炎症参与了阿尔茨海默病(AD)的发展和发病机制。在这里，我们研究了磷虾油的抗炎和抗氧化作用。南极磷虾(Euphausia Superba)是一种南极海洋物种，富含二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)。我们观察了磷虾油饮食(每天80 mg/kg，连续1个月)能否预防内毒素(LPS)(250µg/kg，每天7次)注射所致的AD模型小鼠的淀粉样变性和认知功能障碍，发现磷虾油治疗可抑制内毒素所致的记忆丧失。我们还发现，磷虾油处理抑制了内毒素诱导的诱导型一氧化氮合酶(INOS)和环氧合酶-2(COX-2)的表达，并降低了活性氧(ROS)和丙二醛水平。磷虾油还抑制IκB的降解以及p50和p65转位到内毒素注射的小鼠脑细胞的核中。在抑制神经炎症和氧化应激的同时，磷虾油通过下调APP和BACE1的表达抑制了体内淀粉样β蛋白(1-42)肽的生成。我们发现二十碳五烯酸和二十二碳六烯酸(50和100µM)可剂量依赖性地抑制脂多糖诱导的小胶质细胞BV-2细胞一氧化氮和ROS的生成、COX-2和iNOS的表达以及核因子-κB的活性。这些结果表明，磷虾油通过抗炎、抗氧化和抗淀粉样变性机制改善损伤。
Oxidative stress and neuroinflammation are implicated in the development and pathogenesis of Alzheimer’s disease (AD). Here, we investigated the anti-inflammatory and antioxidative effects of krill oil. Oil from Euphausia superba (Antarctic krill), an Antarctic marine species, is rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). We examined whether krill oil diet (80 mg/kg/day for one month) prevents amyloidogenesis and cognitive impairment induced by intraperitoneal lipopolysaccharide (LPS) (250 µg/kg, seven times daily) injections in AD mice model and found that krill oil treatment inhibited the LPS-induced memory loss. We also found that krill oil treatment inhibited the LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and decreased reactive oxygen species (ROS) and malondialdehyde levels. Krill oil also suppresses IκB degradation as well as p50 and p65 translocation into the nuclei of LPS-injected mice brain cells. In association with the inhibitory effect on neuroinflammation and oxidative stress, krill oil suppressed amyloid beta (1–42) peptide generation by the down-regulating APP and BACE1 expression in vivo. We found that eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (50 and 100 µM) dose-dependently decreased LPS-induced nitric oxide and ROS generation, and COX-2 and iNOS expression as well as nuclear factor-κB activity in cultured microglial BV-2 cells. These results suggest that krill oil ameliorated impairment via anti-inflammatory, antioxidative, and anti-amyloidogenic mechanisms.
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影响因子：
6.3
作者：
Kim DH;Chung JH;Yoon JS;Ha YM;Bae S;Lee EK;Jung KJ;Kim MS;Kim YJ;Kim MK;Chung HY
通讯作者：
Chung HY
影响因子：
4.2
作者：
Grudzien, Aneta;Shaw, Pamela;Mesulam, M. Marsel
通讯作者：
Mesulam, M. Marsel
影响因子：
6.2
作者：
Hartlage-Rübsamen, M;Zeitschel, U;Rossner, S
通讯作者：
Rossner, S
影响因子：
4.5
作者：
Allam-Ndoul B;Guénard F;Barbier O;Vohl MC
通讯作者：
Vohl MC
影响因子：
5.1
作者：
Hwang CJ;Yun HM;Park KR;Song JK;Seo HO;Hyun BK;Choi DY;Yoo HS;Oh KW;Hwang DY;Han SB;Hong JT
通讯作者：
Hong JT