The phytoalexin resveratrol regulates the initiation of hypersensitive cell death in Vitis cell.
The phytoalexin resveratrol regulates the initiation of hypersensitive cell death in Vitis cell.
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DOI：
10.1371/journal.pone.0026405
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Nick P
中科院分区：
文献类型：
--
作者：
Chang X;Heene E;Qiao F;Nick P
白藜芦醇是植物在各种逆境下产生的一种主要的植物抗毒素，具有增强抗病性的作用。北美葡萄藤岩生葡萄的抗性与过敏反应（HR）相关，而感病的欧洲葡萄属酿酒葡萄（vinifera cv.）'黑比诺'不表现出人力资源，但表达基础防御。我们先前已经表明，在来自两个葡萄属物种的细胞系中，细菌效应因子Harpin诱导芪合酶（StSy）转录物的快速和敏感的积累，随后在岩生葡萄中大量细胞死亡。本工作对StSy的产物白藜芦醇的抗植物毒素功能进行了分析。我们找到了那份简历。黑比诺积累的白藜芦醇及其糖苷反式白藜芦醇苷含量较低，而岩生葡萄产生大量的反式白藜芦醇和有毒的氧化δ-葡萄糖苷，表明白藜芦醇的优先代谢在葡萄属的抗性中起作用。细胞对白藜芦醇的反应包括快速碱化、病程相关蛋白5（PR 5）转录物的积累、氧化爆发、肌动蛋白捆绑和细胞死亡。微管破坏和诱导StSy触发Harpin，但不是由白藜芦醇。而大多数反应进行了不同幅度的两个细胞系，白藜芦醇的积累，白藜芦醇诱导的氧化爆发的能力在质量上不同。这些数据导致了一个模型，其中白藜芦醇，除了其作为抗微生物植物抗毒素的经典作用，代表了一个重要的调节器启动HR相关的细胞死亡。
Resveratrol is a major phytoalexin produced by plants in response to various stresses and promotes disease resistance. The resistance of North American grapevine Vitis rupestris is correlated with a hypersensitive reaction (HR), while susceptible European Vitis vinifera cv. ‘Pinot Noir’ does not exhibit HR, but expresses basal defence. We have shown previously that in cell lines derived from the two Vitis species, the bacterial effector Harpin induced a rapid and sensitive accumulation of stilbene synthase (StSy) transcripts, followed by massive cell death in V. rupestris. In the present work, we analysed the function of the phytoalexin resveratrol, the product of StSy. We found that cv. ‘Pinot Noir’ accumulated low resveratrol and its glycoside trans-piceid, whereas V. rupestris produced massive trans-resveratrol and the toxic oxidative δ-viniferin, indicating that the preferred metabolitism of resveratrol plays role in Vitis resistance. Cellular responses to resveratrol included rapid alkalinisation, accumulation of pathogenesis-related protein 5 (PR5) transcripts, oxidative burst, actin bundling, and cell death. Microtubule disruption and induction of StSy were triggered by Harpin, but not by resveratrol. Whereas most responses proceeded with different amplitude for the two cell lines, the accumulation of resveratrol, and the competence for resveratrol-induced oxidative burst differed in quality. The data lead to a model, where resveratrol, in addition to its classical role as antimicrobial phytoalexin, represents an important regulator for initiation of HR-related cell death.
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