An Ocular Commensal Protects against Corneal Infection by Driving an Interleukin-17 Response from Mucosal γδ T Cells.
An Ocular Commensal Protects against Corneal Infection by Driving an Interleukin-17 Response from Mucosal γδ T Cells.
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DOI：
10.1016/j.immuni.2017.06.014
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发表时间：
2017-07-18
期刊：
影响因子：
32.4
通讯作者：
Caspi RR
中科院分区：
文献类型：
--
作者：
St Leger AJ;Desai JV;Drummond RA;Kugadas A;Almaghrabi F;Silver P;Raychaudhuri K;Gadjeva M;Iwakura Y;Lionakis MS;Caspi RR
粘膜部位如肠、口腔、鼻咽和阴道都有相关的口腔植物群。眼睛的表面也是一个粘膜部位，但活的、常驻的眼部微生物组的证据仍然难以捉摸。在这里，我们使用了一个小鼠模型的眼表疾病，以揭示，眼粘膜中存在的，并具有功能性免疫后果的唾液酸。我们分离了一种这样的候选细菌，即乳房棒状杆菌，并表明该生物体从眼粘膜中的γδ T细胞中引发了对局部免疫至关重要的细菌特异性白细胞介素17应答。泪液特异性反应促使中性粒细胞募集和抗菌剂释放到泪液中，并保护眼睛免受致病性白色念珠菌或铜绿假单胞菌感染。我们的研究结果提供了直接的证据，即在眼表上存在常驻的眼内微生物组，并确定了其对眼免疫稳态和宿主防御作用的细胞机制。虽然眼睛是一个粘膜部位，但关于眼表面是否存在常驻微生物组一直存在争议。St.Leger等人表明，生活在结膜上的微生物调节局部粘膜免疫并保护眼睛免受病原体感染。
Mucosal sites such as the intestine, oral cavity, nasopharynx, and vagina all have associated commensal flora. The surface of the eye is also a mucosal site, but proof of a living, resident ocular microbiome remains elusive. Here, we used a mouse model of ocular surface disease to reveal that commensals were present in the ocular mucosa and had functional immunological consequences. We isolated one such candidate commensal, Corynebacterium mastitidis and showed that this organism elicited a commensal-specific interleukin 17 response from γδ T cells in the ocular mucosa that was central to local immunity. The commensal-specfic response drove neutrophil recruitment and the release of antimicrobials into the tears, and protected the eye from pathogenic Candida albicans or Pseudomonas aeruginosa infection. Our findings provide direct evidence that a resident commensal microbiome exists on the ocular surface and identify the cellular mechanisms underlying its effects on ocular immune homeostasis and host defense. Although the eye is a mucosal site, there has been a long-standing controversy regarding whether a resident microbiome exists on the ocular surface. St. Leger et al. show that a microorganism that lives on the conjunctiva tunes local mucosal immunity and protects the eye from pathogenic infection.
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微生物群对铜绿假单胞菌诱导的角膜炎的抗性。
影响因子：
6.7
作者：
Kugadas A;Christiansen SH;Sankaranarayanan S;Surana NK;Gauguet S;Kunz R;Fichorova R;Vorup-Jensen T;Gadjeva M
通讯作者：
Gadjeva M
DOI：
10.1084/jem.184.4.1449
发表时间：
1996-10-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.8
作者：
Lakhundi, Sahreena;Siddiqui, Ruqaiyyah;Khan, Naveed Ahmed
通讯作者：
Khan, Naveed Ahmed
影响因子：
4.4
作者：
Lee, Se Hee;Oh, Doo Hwan;Jeon, Che Ok
通讯作者：
Jeon, Che Ok
影响因子：
9.4
作者：
Eguchi, Hiroshi;Kuwahara, Tomomi;Shiota, Hiroshi
通讯作者：
Shiota, Hiroshi