Serological diagnosis of Mycobacterium avium complex lung diseases by enzyme immunoassay of IgA antibodies against MAC-specific glycopeptidolipid core antigen.
Serological diagnosis of Mycobacterium avium complex lung diseases by enzyme immunoassay of IgA antibodies against MAC-specific glycopeptidolipid core antigen.
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DOI：
10.1016/j.jctube.2020.100189
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发表时间：
2020-12
影响因子：
2
通讯作者：
Kwan CLY
中科院分区：
文献类型：
--
作者：
Tam WO;Wong CF;Wong SSY;Kwan CLY
鸟分枝杆菌复合群肺病（MAC-PD）的发病率在世界范围内呈上升趋势，诊断有时很复杂。最近，一种酶免疫测定（EIA）试剂盒，检测血清伊加抗体对MAC特异性糖肽脂（GPL）核心抗原已被开发出来，并发现是有用的区分MAC-PD从其他肺部疾病。随后还发现该抗体在患有结核分枝杆菌肺病（MAB-PD）的患者中升高。本研究旨在评价该试剂盒对中国香港地区MAC-PD患者的血清学诊断价值。该研究于2017年7月至2018年7月在香港葛量洪医院进行。使用EIA试剂盒对从患有MAC-PD、MAB-PD、肺结核和其他肺部疾病的患者收集的血液样品进行伊加抗体水平的测定。本研究共招募了100例受试者，其中11例被排除。以厂家提供的0.7 U/mL为界值，MAB-PD诊断的敏感性和特异性分别为73.7%和77.6%; MAB-PD诊断的敏感性和特异性分别为50%和77.6%。通过受试者工作特征曲线分析，MAC-PD和MAB-PD的新临界值分别为1.771 U/mL和0.172 U/m。MAB-PD的敏感性和特异性分别为66.7%和72.4%。我们的研究表明，抗MAC特异性糖肽脂核心抗原伊加抗体的酶免疫测定有助于区分MAC和M。香港华人肺结核及其他肺部疾病患者中的肺脓肿。在我们当地人群中进一步进行更大规模的研究，以确定这种抗体检测在诊断和监测MAC和M中的有用性。肺结核可能是有道理的。
There is an increasing trend worldwide in the incidence of Mycobacterium avium complex pulmonary diseases (MAC-PD) and the diagnosis is sometimes complicated. Recently, an enzyme immunoassay (EIA) kit that detects serum IgA antibody against MAC-specific glycopeptidolipid (GPL) core antigen had been developed and found to be useful in discriminating MAC-PD from other lung diseases. The antibody was subsequently also found to be elevated in patients suffering Mycobacterium abscessus pulmonary diseases (MAB-PD). This study is to evaluate this EIA kit in the serological diagnosis of MAC-PD in Hong Kong Chinese patients. The study was conducted in Grantham Hospital, Hong Kong between July 2017 and July 2018. Assay of the IgA antibody level using the EIA kit was done on blood samples collected from patients suffering from MAC-PD, MAB-PD, pulmonary tuberculosis and other lung diseases. There were 100 subjects recruited into the study, among which 11 were excluded. By using the cut-off value 0.7 U/mL provided by the manufacturer, the sensitivity and specificity for diagnosis were 73.7% and 77.6% for MAC-PD; 50% and 77.6% for MAB-PD. By receiver operating characteristic curves analysis, new cut-off for MAC-PD and MAB-PD were calculated as 1.771 U/mL and 0.172 U/m, respectively. The sensitivity and specificity were 68.4% and 86.2% for MAC-PD, whereas 66.7% and 72.4% for MAB-PD. Our study showed that the enzyme immunoassay of IgA antibodies against MAC-specific glycopeptidolipid core antigen could help to distinguishing MAC and M. abscessus pulmonary diseases from pulmonary tuberculosis and other lung diseases among Hong Kong Chinese patients. Further larger scale studies in our local population for the usefulness of this antibody test in the diagnosis and monitoring of MAC and M. abscessus lung diseases might be warranted.
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DOI：
10.5588/ijtld.14.0564
发表时间：
2015-01-01
影响因子：
4
作者：
Kitada, S.;Yoshimura, K.;Kobayashi, K.
通讯作者：
Kobayashi, K.
影响因子：
4.6
作者：
Shibata Y;Horita N;Yamamoto M;Tsukahara T;Nagakura H;Tashiro K;Watanabe H;Nagai K;Nakashima K;Ushio R;Ikeda M;Narita A;Kanai A;Sato T;Kaneko T
通讯作者：
Kaneko T
影响因子：
9.4
作者：
Kitada, Seigo;Maekura, Ryoji;Kobayashi, Kazuo
通讯作者：
Kobayashi, Kazuo
影响因子：
10
作者：
Haworth, Charles S.;Banks, John;Floto, R. Andres
通讯作者：
Floto, R. Andres
影响因子：
11.8
作者：
Nishimura, Tomoyasu;Hasegawa, Naoki;Ishizaka, Akitoshi
通讯作者：
Ishizaka, Akitoshi