Rapid mitochondrial DNA segregation in primate preimplantation embryos precedes somatic and germline bottleneck.
Rapid mitochondrial DNA segregation in primate preimplantation embryos precedes somatic and germline bottleneck.
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DOI：
10.1016/j.celrep.2012.03.011
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发表时间：
2012-05-31
期刊：
影响因子：
8.8
通讯作者：
Mitalipov S
中科院分区：
文献类型：
--
作者：
Lee HS;Ma H;Juanes RC;Tachibana M;Sparman M;Woodward J;Ramsey C;Xu J;Kang EJ;Amato P;Mair G;Steinborn R;Mitalipov S
哺乳动物线粒体DNA（mtDNA）分离和传递的时间和机制知之甚少。女性生殖细胞中的遗传瓶颈被认为是导致异质性mtDNA快速代际分离的主要原因。我们在这里证明，mtDNA分离发生在灵长类动物植入前胚胎发生导致子卵裂球之间的mtDNA变异的分区。在胚胎和来自这些异质胚胎的胚胎干细胞（ESC）中发生了向同质性的实质性转变。我们还观察到广泛的异质性mtDNA变异分布在个别卵母细胞回收这些胎儿。因此，我们在这里提出的证据，以前未知的mtDNA分离和瓶颈在植入前胚胎发育过程中，这表明返回到同质条件可以发生在一个单独的有机体从受精卵到出生的发展过程中，没有通过种系。
The timing and mechanisms of mitochondrial DNA (mtDNA) segregation and transmission in mammals are poorly understood. Genetic bottleneck in female germ cells has been proposed as the main phenomenon responsible for rapid intergenerational segregation of heteroplasmic mtDNA. We demonstrate here that mtDNA segregation occurs during primate preimplantation embryogenesis resulting in partitioning of mtDNA variants between daughter blastomeres. A substantial shift toward homoplasmy occurred in fetuses and embryonic stem cells (ESCs) derived from these heteroplasmic embryos. We also observed a wide range of heteroplasmic mtDNA variants distributed in individual oocytes recovered from these fetuses. Thus, we present here evidence for a previously unknown mtDNA segregation and bottleneck during preimplantation embryo development, suggesting that return to the homoplasmic condition can occur during development of an individual organism from the zygote to birth, without a passage through the germline.
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影响因子：
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作者：
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作者：
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