Aptamers and their biological applications.
Aptamers and their biological applications.
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DOI：
10.3390/s120100612
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Ban C
中科院分区：
文献类型：
--
作者：
Song KM;Lee S;Ban C
近年来，核酸适体受到了众多科学家的关注，因为它不仅具有抗体的所有优点，而且具有独特的优点，如热稳定性好、成本低、应用范围广等。在这篇综述中，我们提出了为什么适体被称为抗体的替代品的原因。此外，几种类型的体外选择过程，包括硝酸纤维素膜过滤，亲和色谱，磁珠，和毛细管电泳为基础的选择方法，详细解释。我们还介绍了适体在疾病诊断和小分子检测中的各种应用。许多分析技术，如电化学，比色，光学和质量敏感的方法，可以用来检测目标，由于方便的修改和适体的稳定性。最后，介绍了适体的几种医学和分析应用。总之，适体是用于不同领域的有前途的材料，不仅作为抗体的替代品，而且作为医疗和分析设备的核心组件。
Recently, aptamers have attracted the attention of many scientists, because they not only have all of the advantages of antibodies, but also have unique merits, such as thermal stability, low cost, and unlimited applications. In this review, we present the reasons why aptamers are known as alternatives to antibodies. Furthermore, several types of in vitro selection processes, including nitrocellulose membrane filtration, affinity chromatography, magnetic bead, and capillary electrophoresis-based selection methods, are explained in detail. We also introduce various applications of aptamers for the diagnosis of diseases and detection of small molecules. Numerous analytical techniques, such as electrochemical, colorimetric, optical, and mass-sensitive methods, can be utilized to detect targets, due to convenient modifications and the stability of aptamers. Finally, several medical and analytical applications of aptamers are presented. In summary, aptamers are promising materials for diverse areas, not just as alternatives to antibodies, but as the core components of medical and analytical equipment.
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DOI：
10.3390/s100504541
发表时间：
2010
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Han K;Liang Z;Zhou N
通讯作者：
Zhou N
影响因子：
16.6
作者：
Elowe, Nadine H.;Nutiu, Razvan;Brown, Eric A.
通讯作者：
Brown, Eric A.
影响因子：
8.6
作者：
Habenicht, Bradley F.;Prezhdo, Oleg V.
通讯作者：
Prezhdo, Oleg V.
影响因子：
12.6
作者：
Blake, DA;Jones, RM;Yu, HN
通讯作者：
Yu, HN
适体介导的siRNA传递。
影响因子：
14.9
作者：
Chu, Ted C.;Twu, Karen Y.;Ellington, Andrew D.;Levy, Matthew
通讯作者：
Levy, Matthew