Role of Endogenous Opiates in Glucoprivic Inhibition of the Luteinizing Hormone Surge and Fos Expression by Preoptic Gonadotropin‐Releasing Hormone Neurones in Ovariectomized Steroid‐Primed Female Rats
Role of Endogenous Opiates in Glucoprivic Inhibition of the Luteinizing Hormone Surge and Fos Expression by Preoptic Gonadotropin‐Releasing Hormone Neurones in Ovariectomized Steroid‐Primed Female Rats
复制标题
内源性阿片类药物在去势类固醇激素雌性大鼠视前促性腺激素释放激素神经元对黄体生成素激增和 Fos 表达的抑制中的作用
DOI：
--
复制
发表时间：
1998
影响因子：
3.2
通讯作者：
Sylvester
中科院分区：
文献类型：
--
作者：
Briski;Sylvester
在雌性哺乳动物中，排卵前黄体生成素（LH）“激增”引发排卵和随后的卵泡向黄体的转化，因此是成功繁殖的关键组成部分。鉴于有证据表明热量限制会损害这种激增，接下来的实验利用药理学策略来确定葡萄糖底物稳态是否会影响这一关键激素事件的大小和/或持续时间。在预期的黄体生成素激增开始之前，将雌激素和孕激素启动的卵巢切除（OVX）大鼠静脉注射葡萄糖抗代谢物，2 -脱氧- d -葡萄糖（2DG: 100或400 mg/kg），或对照物，生理盐水。其余大鼠分别用2DG （100 μg/只）或生理盐水经脑室灌胃预处理。虽然葡萄糖活化并没有消除这些动物的下午LH激增，但相对于他们的载体注射对照组，注射较高剂量的2DG或通过体外注射途径使用该药物的组，平均血浆LH水平显著降低。在其他研究中，静脉给药阿片受体拮抗剂纳曲酮（NALT）部分逆转了2DG对性腺类固醇诱导的LH激增的抑制作用。双标记免疫细胞化学显示，在促性腺激素释放激素（GnRH）免疫阳性神经元亚群中，促性腺激素释放激素（GnRH）免疫阳性神经元亚群中，促性腺激素释放激素（LH）的最大排卵期释放之前，OVX的视前区和下丘脑前部组织切片显示出核Fos -免疫反应性（- ir）。用2DG i.c.v预处理的动物显示，表现出Fos - ir的GnRH神经元的平均数量显著减少，而2DG和NALT的共同施用导致双标记神经元的数量与未药物处理的对照组相似。这些研究表明，葡萄糖底物失衡会降低黄体生成素激增的幅度，这种代谢挑战对生殖神经内分泌轴的调节作用与性腺类固醇正反馈对视前GnRH神经元转录激活的改变有关。目前的研究结果也支持中枢阿片能神经传递在动物模型中对黄体生成素分泌的葡萄糖调节中的作用。
In female mammals, the preovulatory luteinizing hormone (LH) ‘surge’ elicits ovulation and the subsequent transformation of Graafian follicles into corpora lutea, and is thus a critical component of successful reproduction. In light of evidence that this surge is impaired as a consequence of caloric restriction, the following experiments utilized pharmacological strategies to determine whether glucose substrate homeostasis influences the magnitude and/or duration of this pivotal hormonal event. Groups of oestrogen‐and progesterone‐primed ovariectomized (OVX) rats were injected intravenously (i.v.) with the glucose antimetabolite, 2‐deoxy‐d‐glucose (2DG: 100 or 400 mg/kg), or the vehicle, saline, prior to onset of the expected LH surge. Other rats were pretreated with 2DG (100 μg/rat) or saline by an intracerebroventricular (i.c.v) route. While glucoprivation did not abolish the afternoon LH surge in these animals, mean plasma LH levels were significantly decreased in groups injected with the higher i.v. dose of 2DG or treated with this drug by an i.c.v route, relative to their vehicle‐injected controls. In other studies, i.c.v delivery of the opioid receptor antagonist, naltrexone (NALT), partially reversed the inhibitory effects of 2DG on the gonadal steroid‐induced LH surge. Dual‐label immunocytochemistry of tissue sections from the preoptic area and anterior hypothalamus of OVX, steroid‐primed rats revealed nuclear Fos‐immunoreactivity (‐ir) in a subpopulation of gonadotropin‐releasing hormone‐(GnRH‐)immunopositive neurones prior to maximal preovulatory LH release. Animals pretreated with 2DG i.c.v showed a significant decrease in mean numbers of GnRH neurones exhibiting Fos‐ir, whereas coadministration of 2DG and NALT resulted in numbers of double‐labelled neurones that were similar to those detected in the non‐drug‐treated controls. These studies show that magnitude of the LH surge is decreased by glucose substrate imbalance, and that regulatory effects of this metabolic challenge on the reproductive neuroendocrine axis is correlated with alterations in the transcriptional activation of preoptic GnRH neurones by gonadal steroid positive feedback. The present results also support a role for central opiatergic neurotransmission in glucoprivic regulation of cyclic LH secretion in this animal model.
登录
查看更多内容
DOI：
10.1210/endo.137.2.8593808
发表时间：
1996
期刊：
Endocrinology.
影响因子：
--
作者：
Bucholtz,DC;Vidwans,NM;Herbosa,CG;Schillo,KK;Foster,DL
通讯作者：
Foster,DL
胰岛素诱导的叙利亚仓鼠不发情。
DOI：
10.1152/ajpregu.1991.260.1.r148
发表时间：
1991
期刊：
The American journal of physiology
影响因子：
--
作者：
Wade,GN;Schneider,JE;Friedman,MI
通讯作者：
Friedman,MI
通过限制葡萄糖可用性来抑制黄体生成素脉冲是由脑干中的传感器介导的。
DOI：
10.1210/endo.137.4.8625886
发表时间：
1996
期刊：
Endocrinology.
影响因子：
--
作者：
Murahashi,K;Bucholtz,DC;Nagatani,S;Tsukahara,S;Tsukamura,H;Foster,DL;Maeda,KI
通讯作者：
Maeda,KI
结合免疫细胞化学和原位杂交组织化学的三重标记方法：证明雌性大鼠黄体生成素释放激素神经元的 Fos 免疫反应性和甘丙肽 mRNA 表达亚群之间的重叠。
DOI：
10.1177/43.4.7534782
发表时间：
1995
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Hrabovszky,E;Vrontakis,ME;Petersen,SL
通讯作者：
Petersen,SL
发情前、卵巢切除和类固醇治疗的大鼠弓状核中阿黑皮素原基因表达的昼夜模式：在周期性黄体生成素分泌中的可能作用。
DOI：
10.1210/mend-4-6-886
发表时间：
1990
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Wise,PM;Scarbrough,K;Weiland,NG;Larson,GH
通讯作者：
Larson,GH