Microbial products induce claudin-2 to compromise gut epithelial barrier function.
Microbial products induce claudin-2 to compromise gut epithelial barrier function.
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微生物产物诱导Claudin-2损害肠道上皮屏障功能。
DOI：
10.1371/journal.pone.0068547
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Liu X;Yang G;Geng XR;Cao Y;Li N;Ma L;Chen S;Yang PC;Liu Z
上皮功能障碍是许多疾病中的重要致病性这项研究通过cldn2培养模型观察到了CLDN2的cldn2在小鼠的上皮层和食物过敏的患者中的表达通过记录跨性别分子示踪剂的渗透性来评估肠上皮单层中的屏障功能。小鼠在敏感的小鼠中染色，以及食物过敏的患者。在整个上皮屏障中，CLDN2阻止了与过敏性的超敏反应。
The epithelial barrier dysfunction is an important pathogenic feature in a number of diseases. The underlying mechanism is to be further investigated. The present study aims to investigate the role of tight junction protein claudin-2 (Cldn2) in the compromising epithelial barrier function. In this study, the expression of Cldn2 in the epithelial layer of mice and patients with food allergy was observed by immunohistochemistry. The induction of Cldn2 was carried out with a cell culture model. The Cldn2-facilitated antigen internalization was observed by confocal microscopy. The epithelial barrier function in the gut epithelial monolayer was assessed by recording the transepithelial resistance and assessing the permeability to a macromolecular tracer. The results showed that the positive immune staining of Cldn2 was observed in the epithelial layer of the small intestine that was weakly stained in naïve control mice, and strongly stained in sensitized mice as well as patients with food allergy. Exposure to cholera toxin or Staphylococcal enterotoxin B induced the expression of Cldn2 in HT-29 or T84 cells. Cldn2 could bind protein antigen to form complexes to facilitate the antigen transport across the epithelial barrier. Blocking Cldn2 prevented the allergen-related hypersensitivity the intestine. We conclude that the tight junction protein Cldn2 is involved in the epithelial barrier dysfunction.
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影响因子：
4.3
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通讯作者：
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14.2
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