Purine receptors modulate chondrocyte extracellular inorganic pyrophosphate production.
Purine receptors modulate chondrocyte extracellular inorganic pyrophosphate production.
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DOI：
10.1016/j.joca.2010.08.004
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发表时间：
2010-11
影响因子：
7
通讯作者：
Ryan LM
中科院分区：
文献类型：
--
作者：
Rosenthal AK;Hempel D;Kurup IV;Masuda I;Ryan LM
Extracellular inorganic pyrophosphate (ePPi) plays a key role in the regulation of normal and pathologic mineralization. The purpose of this work was to evaluate the role of P1 and P2 purine receptors in modulating ePPi production by articular chondrocytes. Porcine cartilage explants and chondrocyte monolayers were cultured in the presence of P1 agonists, or a P2 agonist or antagonist and inhibitors of P2 signaling. Ambient media ePPi concentrations were measured after 48 to 96 hours. The P1 agonists NECA and CGS 21680 significantly decreased ePPi concentrations surrounding chondrocytes and cartilage explants. The P2 agonist, ADP, increased ePPi levels, and the P2 antagonist, suramin, decreased ePPi concentrations. Thapsigargin and BAPTA, which dampen Ca2+-related P2 signaling, suppressed the response to ADP. Purine receptors are important regulators of ePPi production by chondrocytes. P1 receptor stimulation diminishes and P2 receptor stimulation enhances ePPi production. Alterations in receptor signaling or aberrations of extracellular purine nucleotide metabolism resulting in abnormal quantities or proportions of P1 and P2 receptor ligands could foster changes in ePPi production that in turn affect mineralization. We propose a homeostatic role for extracellular purine nucleotides and purine receptors in stabilizing ePPi concentrations.
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DOI：
10.1006/bbrc.1994.1314
发表时间：
1994-03-15
影响因子：
3.1
作者：
IWAMOTO, T;UMEMURA, S;ISHII, M
通讯作者：
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DOI：
10.1073/pnas.142063399
发表时间：
2002-07-09
影响因子：
11.1
作者：
Hessle, L;Johnson, KA;Millán, JL
通讯作者：
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DOI：
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2000-07-03
期刊：
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM
影响因子：
--
作者：
Hamilton, SG;McMahon, SB
通讯作者：
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影响因子：
2.9
作者：
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通讯作者：
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影响因子：
4.1
作者：
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通讯作者：
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