Modulation of Viscoelasticity and HIV Transport as a Function of pH in a Reversibly Crosslinked Hydrogel.
Modulation of Viscoelasticity and HIV Transport as a Function of pH in a Reversibly Crosslinked Hydrogel.
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DOI：
10.1002/adfm.200900757
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发表时间：
2009-09-23
影响因子：
19
通讯作者：
Kiser, Patrick F.
中科院分区：
文献类型：
--
作者：
Jay, Julie I.;Shukair, Shetha;Langheinrich, Kristofer;Hanson, Melissa C.;Cianci, Gianguido C.;Johnson, Todd J.;Clark, Meredith R.;Hope, Thomas J.;Kiser, Patrick F.
对生理刺激作出反应的材料在开发用于现代治疗的先进生物材料方面非常重要。苯基硼酸酯(PBA)和水杨基羟基甲酸酯(SHA)形成的共价交联剂的可逆性被用来提供一种用于阴道的pH响应性凝胶。动态流变学表明，凝胶的频率相关粘弹性性质受pH的调制。在pH为4.8时，粘性成分在大部分频率范围内占主导地位。随着pH值的增加，特征弛豫时间继续增加，而平台在pH 6以上下降。在pH 7.5时，弹性成分在整个扫频过程中占主导地位，并且主要与频率无关。粒子跟踪评估了荧光标记的HIV-1和100 nm乳胶粒子在PBA-SHA交联凝胶中的传输随pH的变化。在pH 4.8时，滞后时间大于3秒的总体平均平方位移表明，艾滋病毒-1和100 nm颗粒的运输受到基质的显著阻碍，扩散系数小于0.0002 µm~2s−1。因此，这种pH响应凝胶具有显著减少艾滋病毒-1向敏感组织的运输的潜力，从而防止艾滋病毒-1从男性传播到女性的第一阶段。
Materials that respond to physiological stimuli are important in developing advanced biomaterials for modern therapies. The reversibility of covalent crosslinks formed by phenylboronate (PBA) and salicylhydroxamate (SHA) has been exploited to provide a pH‐responsive gel for application to the vaginal tract. Dynamic rheology reveals that the gel frequency‐dependent viscoelastic properties are modulated by pH. At pH 4.8 the viscous component dominates throughout most of the frequency range. As the pH increases, the characteristic relaxation time continues to increase while theG′Plateaulevels off above pH 6. At pH 7.5, the elastic component dominates throughout the frequency sweep and is predominately independent of frequency. Particle tracking assesses the transport of both fluorescently labeled HIV‐1 and 100‐nm latex particles in the PBA–SHA crosslinked gel as a function of pH. At pH 4.8 the ensemble‐averaged mean squared displacement at lag times greater than three seconds reveals that transport of the HIV‐1 and 100‐nm particles becomes significantly impeded by the matrix, exhibiting diffusion coefficients less than 0.0002 µm2s−1. This pH‐responsive gel thus displays properties that have the potential to significantly reduce the transport of HIV‐1 to susceptible tissues and thus prevent the first stage of male‐to‐female transmission of HIV‐1.
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影响因子：
32.4
作者：
Hladik, Florian;Sakchalathorn, Polachai;Ballweber, Lamar;Lentz, Gretchen;Fialkow, Michael;Eschenbach, David;McElrath, M. Juliana
通讯作者：
McElrath, M. Juliana
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通讯作者：
Hope, Thomas J.
影响因子：
19
作者：
Levy, Tatjana;Dejugnat, Christophe;Sukhorukov, Gleb B.
通讯作者：
Sukhorukov, Gleb B.