Cryptosporidium parvum Infection Depletes Butyrate Producer Bacteria in Goat Kid Microbiome.
Cryptosporidium parvum Infection Depletes Butyrate Producer Bacteria in Goat Kid Microbiome.
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DOI：
10.3389/fmicb.2020.548737
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发表时间：
2020
影响因子：
5.2
通讯作者：
Adjou KT
中科院分区：
文献类型：
--
作者：
Mammeri M;Obregón DA;Chevillot A;Polack B;Julien C;Pollet T;Cabezas-Cruz A;Adjou KT
微小隐孢子虫是一种感染反刍动物和人类的重要顶端复合体寄生虫。我们研究了细小弧菌感染对山羊羔羊微生物群的影响。对未经寄生虫感染的山羊口服细小隐孢子虫，用免疫荧光法和定量聚合酶链式反应检测粪便样本中的感染情况，持续26天。年龄匹配的山羊作为未感染的对照。与未感染的对照相比，感染的山羊的体重增加、腹泻和脱水都有所减少。感染后5天细菌多样性降低，但在感染后15天恢复。感染改变了几个分类群的相对丰度。在5dpi和15dpi处，共有38个分类群在对照山羊和感染山羊之间显示出显著的丰度差异。共生网络分析表明，感染导致了不同的类群相互作用模式，而微小弧菌感染增加了特定类群的相对丰度。16S数据集用于使用软件包PICRUSt2进行元基因组预测。在感染5dpi和15dpi时，分别有34条和40条MetaCyc通路(总共387条)受到感染的显著影响。值得注意的是，微小隐孢子虫减少了细菌中丁酸产生途径的丰度。低水平的丁酸可能会增加粘膜炎症和组织修复。我们的结果表明，微小隐孢子虫感染引起的肠道炎症与丁酸产生菌的减少有关。这一见解可能是开发新的控制策略以改善动物健康的基础。
Cryptosporidium parvum is an important apicomplexan parasite infecting ruminants and humans. We characterized the impact of C. parvum infection on the goat kid microbiome. C. parvum was orally administered to parasite-naïve goats, and infection was monitored for 26 days in fecal samples using immunofluorescence assay and qPCR tests. Age-matched goats served as uninfected controls. A reduction in body weight gain, diarrhea, and dehydration were observed in infected goats compared to the uninfected controls. Infection decreased the bacterial diversity 5 days post-infection (dpi), but this parameter recovered at 15 dpi. The infection altered the relative abundance of several taxa. A total of 38 taxa displayed significant differences in abundance between control and infected goats at both 5 and 15 dpi. Co-occurrence network analysis revealed that the infection resulted in a differential pattern of taxa interactions and that C. parvum infection increased the relative abundance of specific taxa. The 16S data set was used for metagenome predictions using the software package PICRUSt2. As many as 34 and 40 MetaCyc pathways (from 387 total) were significantly affected by the infection at 5 and 15 dpi, respectively. Notably, C. parvum decreased the abundance of butyrate-producing pathways in bacteria. Low levels of butyrate may increase mucosal inflammation and tissue repair. Our results suggest that the gut inflammation induced by C. parvum infection is associated with the reduction of butyrate-producing bacteria. This insight could be the basis for the development of novel control strategies to improve animal health.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
6.4
作者：
Kirkpatrick, BD;Daniels, MM;Sears, CL
通讯作者：
Sears, CL
DOI：
10.1016/s0749-0720(15)30838-0
发表时间：
1990-11-01
影响因子：
4.1
作者：
FOREYT, WJ
通讯作者：
FOREYT, WJ
影响因子：
4.9
作者：
Burdet, Charles;Sayah-Jeanne, Sakina;de Gunzburg, Jean
通讯作者：
de Gunzburg, Jean
影响因子：
3.1
作者：
Giadinis, Nektarios D;Papadopoulos, Elias;Karatzias, Harilaos
通讯作者：
Karatzias, Harilaos