Development of a generic microfluidic device for simultaneous detection of antibodies and nucleic acids in oral fluids.
Development of a generic microfluidic device for simultaneous detection of antibodies and nucleic acids in oral fluids.
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DOI：
10.1155/2013/543294
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发表时间：
2013
影响因子：
--
通讯作者：
Corstjens PL
中科院分区：
文献类型：
--
作者：
Chen Z;Abrams WR;Geva E;de Dood CJ;González JM;Tanke HJ;Niedbala RS;Zhou P;Malamud D;Corstjens PL
A prototype dual-path microfluidic device (Rheonix CARD) capable of performing simultaneously screening (antigen or antibody) and confirmatory (nucleic acid) detection of pathogens is described. The device fully integrates sample processing, antigen or antibody detection, and nucleic acid amplification and detection, demonstrating rapid and inexpensive “sample-to-result” diagnosis with performance comparable to benchtop analysis. For the chip design, a modular approach was followed allowing the optimization of individual steps in the sample processing process. This modular design provides great versatility accommodating different disease targets independently of the production method. In the detection module, a lateral flow (LF) protocol utilizing upconverting phosphor (UCP) reporters was employed. The nucleic acid (NA) module incorporates a generic microtube containing dry reagents. Lateral flow strips and PCR primers determine the target or disease that is diagnosed. Diagnosis of HIV infection was used as a model to investigate the simultaneous detection of both human antibodies against the virus and viral RNA. The serological result is available in less than 30 min, and the confirmation by RNA amplification takes another 60 min. This approach combines a core serological portable diagnostic with a nucleic acid-based confirmatory test.
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影响因子：
16
作者：
Park, Seungkyung;Zhang, Yi;Lin, Shin;Wang, Tza-Huei;Yang, Samuel
通讯作者：
Yang, Samuel
影响因子：
9.4
作者：
Corstjens, Paul L. A. M.;van Lieshout, Lisette;van Dam, Govert J.
通讯作者：
van Dam, Govert J.
影响因子：
9.4
作者：
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通讯作者：
FENYO, EM
DOI：
10.1073/pnas.0604663103
发表时间：
2006-12-19
影响因子：
11.1
作者：
Easley, Christopher J.;Karlinsey, James M.;Landers, James P.
通讯作者：
Landers, James P.
DOI：
10.1097/igc.0b013e31825529b7
发表时间：
2012-07-01
影响因子：
4.8
作者：
Jentschke, Matthias;Soergel, Philipp;Hillemanns, Peter
通讯作者：
Hillemanns, Peter