Radiation-induced DNA damage and repair in quiescent and proliferating human tumor cells in vitro.
Radiation-induced DNA damage and repair in quiescent and proliferating human tumor cells in vitro.
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体外静止和增殖的人类肿瘤细胞中辐射诱导的 DNA 损伤和修复。
DOI：
10.1080/09553008914550431
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发表时间：
1989
影响因子：
2.6
通讯作者：
Keng,PC
中科院分区：
文献类型：
--
作者：
Wilson,KM;Keng,PC
本研究的目的是检测体外培养的静止期和增殖期人肿瘤细胞中辐射诱导的DNA链断裂和修复，并确定它们与辐射敏感性和潜在致死性损伤修复（PLDR）的关系。使用离心淘洗，我们已经从HEp-3人鳞状细胞癌细胞的饲养平台期培养物中分离出相对纯的静止和增殖细胞群。这通过[3 H]-胸苷标记和流式细胞术的吖啶橙子（AO）染色证实。静止期细胞对电离辐射的敏感性（D 0 = 0·97戈伊）高于增殖期细胞（D 0 = 1·28戈伊）。然而，静止细胞表现出更高的潜在致死性损伤（PLDR）的修复比增殖细胞。单链断裂（ssb）和双链断裂（dsb）的修复，如通过过滤器洗脱测量，在静止和增殖细胞之间没有显着差异。对于这两个群体，SSB和DSB的修复动力学和最终的损伤是相同的，这表明DNA链断裂的修复并不完全负责静止和增殖细胞之间的辐射敏感性的差异。
The purpose of this study was to examine radiation-induced DNA strand breakage and repair in quiescent and proliferating human tumor cellsin vitroand determine their relationship to radiation sensitivity and potentially lethal damage repair (PLDR). Using centrifugal elutriation we have isolated from fed plateau-phase cultures of HEp-3 human squamous carcinoma cells, relatively pure populations of quiescent and proliferating cells. This was confirmed by both [3H]-thymidine labelling and acridine orange (AO) staining with flow cytometry. Quiescent cells were more sensitive to ionizing radiation (D0= 0·97 Gy) than were proliferating cells (D0= 1·28 Gy). However, quiescent cells showed higher repair of potentially lethal damage (PLDR) than did proliferating cells. Repair of single-strand breaks (ssb) and double-strand breaks (dsb) as measured by filter elution did not differ significantly between quiescent and proliferating cells. For both populations, ssb and dsb repair kinetics and final damage remaining were the same, suggesting that repair of DNA strand breaks is not entirely responsible for the difference in radiation sensitivity between quiescent and proliferating cells.
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培养中的静止小鼠乳腺癌细胞的 DNA 损伤修复。
DOI：
10.1016/0167-4781(85)90043-0
发表时间：
1985
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Warters,RL;Lyons,BW;Ridinger,DN;Dethlefsen,LA
通讯作者：
Dethlefsen,LA
可的松处理的实验动物中维持的可移植人类肿瘤：H.S.
DOI：
--
发表时间：
2006
期刊：
影响因子：
--
作者：
Frank M. Collins;G. Mackaness;R. Blanden
通讯作者：
R. Blanden
在 X 射线照射的有丝分裂和 G1 期 CHO 细胞中，诱导的 DNA 双链断裂水平与细胞杀伤无关。
DOI：
10.1080/09553008814552531
发表时间：
1988
期刊：
International journal of radiation biology and related studies in physics, chemistry, and medicine
影响因子：
--
作者：
Iliakis,G;Okayasu,R
通讯作者：
Okayasu,R
营养状态对从异质人类结肠癌获得的两个肿瘤亚群中辐射损伤表达的影响。
DOI：
10.1016/0360-3016(86)90416-5
发表时间：
1986
期刊：
International journal of radiation oncology, biology, physics
影响因子：
--
作者：
C. Arundel;J. Leith
通讯作者：
J. Leith
影响因子：
3.4
作者：
LITTLE, JB
通讯作者：
LITTLE, JB