Ontogeny of angiotensin type 2 and type 1 receptor expression in mice.
Ontogeny of angiotensin type 2 and type 1 receptor expression in mice.
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DOI：
10.1177/1470320312443720
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发表时间：
2012-09
期刊：
影响因子：
--
通讯作者：
Gao L
中科院分区：
文献类型：
--
作者：
Gao J;Chao J;Parbhu KJ;Yu L;Xiao L;Gao F;Gao L
在当前的实验中，我们通过蛋白质印迹分析确定了发育正常小鼠中 AT2R 和 AT1R 蛋白的表达。结果表明，（1）在所有检测到的脑区和脊髓中，与胎儿和新生儿相比，成年小鼠的总蛋白提取物中AT2R表达显着升高，AT1R表达显着降低； (2)其他主要器官，包括心、肺、肝、肾，表现出与脑和脊髓相同的表达模式； (3)从产前1天到6周龄的小鼠脑干总蛋白提取物中发现AT2R和AT1R表达的相互变化。 AT2R与AT1R蛋白表达量呈负相关； (4) 在脑干的膜和细胞质部分中，成年小鼠比胎儿和新生儿表现出更高的 AT2R 表达和更低的 AT1R 表达； (5)在脑干中，胎儿、新生和成年小鼠的AT2R和AT1R mRNA水平没有显着差异。上述结果再次证实了我们之前在大鼠中的发现，即与胎儿和新生儿相比，成年动物具有更高的 AT2R 表达和更低的 AT1R 表达。这些数据表明 AT1R 参与胎儿发育，AT2R 参与成人功能。
In the current experiment, we determined AT2R and AT1R protein expression by Western blot analysis in developing normal mice. The results indicate that, (1) in all detected brain regions and in the spinal cord, adult mice exhibited significantly higher AT2R expression and lower AT1R expression in total protein extracts compared to fetuses and neonates; (2) other major organs, including heart, lung, liver, and kidney, exhibited the same expression pattern as the brain and spinal cord; (3) reciprocal changes in AT2R and AT1R expression were found in the total protein extracts from the brainstems of mice from one day prenatal to six weeks of age. There was a negative correlation between AT2R and AT1R protein expression; (4) in both membrane and cytosolic fractions from the brainstem, adult mice exhibited higher AT2R and lower AT1R expression than did fetuses and neonates; (5) in the brainstem, there were no significant differences in AT2R and AT1R mRNA levels among fetal, neonatal, and adult mice. The above results reconfirmed our previous finding in rats that adult animals have higher AT2R and lower AT1R expression compared to fetuses and neonates. These data imply an involvement of AT1R in fetal development and of AT2R in adult function.
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